Hymenoptera sting reactions in southern Italy forestry workers: our experience compared to reported data
© The Author(s) 2018
Received: 31 July 2017
Accepted: 24 March 2018
Published: 17 April 2018
Hymenoptera sting reactions are among life-threatening causes of allergy. Several epidemiology studies have assessed the risk of these kind of reactions, among the general population, around 3% of adults. This incidence increases among highly at risk populations such as outdoor workers. Hymenoptera stings among forestry workers (FW) are occupational triggers but it has not yet been well defined which is the real incidence of anaphylaxis in these workers, not even in Italy. Two Italian studies reported on the risk of hymenoptera stings (HS) in northern Italy (NI) and central Italy (CI) FW while no data is available on the prevalence in southern Italy (SI) ones.
A population of 341 SI FW (301 males and 40 females, mean age 51 years, range 43–63 years), who worked in Sicily, was investigated submitting a standardized questionnaire dealing with reactions to Hymenoptera stings, such as large local reactions (LLR) and systemic reactions (SR).
HS occurred in 203 FW (59%) and caused reactions in 77 (22%); LLR occurred in 46 (13%) and SR in 31 (9%); SR were life threatening in 9/341 (3%) FW and were treated with epinephrine at the emergency unit as workers did not carry an epinephrine auto-injector. A SR at a subsequent HS followed a LLR in 21/46 FW (46%).
FW in SI have a generic risk of HS anaphylaxis as in the general population but a higher risk of SR and LLR respect to forestry populations from different Italian geographical areas.SR among SI FW occurred in 9% of them, while published data report the incidence of SR around 2 and 4%, respectively, in the Centre and North Italy FW. The incidence of LLR in SI FW was also higher (13%) than in CI (2%) and NI (10%) ones. Previous LLR in our SI population represented a high risk factor for developing a SR and therefore a red flag for future anaphylaxis and prescription of an epinephrine auto-injector.
Hymenoptera stings (HS), even if in the vast majority cause only minor problems, account, even nowadays in the third millennium, for deaths usually resulting from immunologic mechanisms.
Self-reported systemic HS reactions among adults range from 0.5 to 3.3% in the US  while in Europe studies report the prevalence of systemic reactions (SR) between 0.3 and 7.5% ; mortality due to HS has been reported ranging from 0.03 to 0.48 fatalities per 1,000,000 population per year .
Quality of life of subjects who have experienced a SR after a HS is impaired as these subjects usually develop emotional distress during day life . Furthermore, HS are among the commonest triggers of occupational anaphylaxis especially in outdoor workers such as beekeepers , gardeners , farmers, truck drivers, masons  and forestry workers (FW) . Some authors investigated the prevalence of reactions to HS among FW. Japanese FW have a percentage of SR to HS significantly higher than control subjects do . Incorvaia  and Copertaro  studied northern Italy (NI) and central Italy (CI) populations of FW, respectively in order to evaluate the prevalence of HS. Up to now, no data is available on southern Italy (SI) FWs’ risk of HS reactions.
We carried out an observational retrospective study on a population of FW from Sicily, a SI region, submitting a standardized fully anonymous questionnaire dealing with reactions to HS.
The reactions to HS were classified into large local reactions (LLR), defined as a swelling exceeding a diameter of 10 cm that lasted longer than 24 h, or SR according to Mueller’s classification  with skin, gastrointestinal, respiratory and cardiovascular systems’ involvement. Life threatening SR, defined as anaphylaxis, were the reactions characterized by a rapid onset of airway, breathing, circulatory, or gastrointestinal problems defined according to EAACI and WAO Guidelines [13, 14].
A physician administered a questionnaire to the FW in order to collect information about age, sex, HS, stinging insect, average number of stings respect to how long they had been working, frequency of stinging, degree of reaction to a HS.
The population of FW consisted of 341 workers, mean age 51 years (range 43–63 years), 301 males and 40 females; HS occurred in 203 FW (59%), all during working hours. The culprit Hymenoptera, recognized by each stung FW, was a Vespid in 108 and an Apid in nine workers. Stings received by the other FW were most likely from Vespids as they did nor remember to have removed the sting. In average FW included in the study had been working for 23 years. Since their employment, 64 workers had received from 1 to 3 stings, 86 between 3 and 5 while 53 more than 5. HS reactions occurred in 77 FW (22%). LLR occurred in 46 FW (13%) and about half of them, 21 (46%), after a second sting in a further occasion, had a SR. LLR were also more frequent in workers who medially had been working for more years. The overall number of FW who had a SR was of 31/341 (9%).
These reactions had been treated with topical or systemic corticosteroids or antihistamines. SR were life threatening in 9/341 (3%) and were treated with epinephrine at the emergency unit together with systemic antihistamines such as clorpheniramine and corticosteroids as methylprednisolone. Furthermore, all the workers who had life-threatening SR were among those who had received more than five stings. No FW carried an epinephrine auto-injector.
FW are at high risk of HS and may develop occupation-related allergies but rarely surveys on the natural history of HS, among these or other outdoor workers, are reported .
General data and kind of reaction to Hymenoptera stings in FW from SI (current report), CI and NI (as previously published 10, 11)
HS occurred in 203/341 SI FW (59%) compared to 76/112 (68%) in NI and 179/206 (87%) in CI ones. Nonetheless, among SI FW, only 64 workers received no more than three stings while 86 from 3 to 5 and 53 more than 5. The higher incidence of both systemic and LLR in SI FW could be correlated to the shortness of interval between stings . Hymenoptera allergy is one of the allergic disease problems related to climate change which is involving also Sicily . A warming climate can cause dramatic shifts on these insects’ populations from extinction but usually to overpopulation with a significant increase in the number of people seeking care for stings .
The high incidence of LLR in our FW population is unusually high compared to the general population as previously reported  but up to now we are unable to explain this singularity. Only in highly exposed subjects, such as beekeepers  or subjects from a rural population in the Mediterranean area  a prevalence has been reported. It must be underlined that Sicilian FW, such as the population we examined, work in a similar geographical area.
Our data confirm what already reported in literature on how strong impact, hymenoptera venom allergy, has on work causing in some cases work disability .
As far as LLR in SI FW, they not also occurred with a higher incidence (13%) but a SR followed at a subsequent HS (21/46) in a high percentage of FW (46%).
LLR in SI FW represented a high risk factor for developing a SR and consequently a red flag for future anaphylaxis with the need of an epinephrine auto-injector prescription beforehand . This accounts for the suggestion of a thorough allergy screening and follow-up in subjects with a high occupational risk of Hymenoptera stinging.
All the authors actively contributed in the manuscript. LR wrote the manuscript, SG, drafted the study, MG and GS submitted the questionnaire, FP and GC analyzed data from the questionnaire. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Availability of data and materials
Data collected with the questionnaire are available.
Consent for publication
Ethics approval and consent to participate
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Golden DB. Anaphylaxis to insect stings. Immunol allergy Clin North Am. 2015;35:287–302.View ArticlePubMedGoogle Scholar
- Bilò B, Rueff F, Mosbech H, Bonifazi F, Oude_Elmerink JN, EAACI Interest group on Insect Venom hypersensitivity. Diagnosis of hymenoptera venom allergy. Allergy. 2005;60:1339–49.View ArticlePubMedGoogle Scholar
- Pastorello EA, Rivolta F, Bianchi M, Mauro M, Pravettoni V. Incidence of anaphylaxis in the emergency department of a general hospital in Milan. J Chromatogr B Biomed Sci Appl. 2001;756:11–7.View ArticlePubMedGoogle Scholar
- Paolucci G, Folletti I, Toren K, Muzi G, Murgia N. Hymenoptera venom allergy: work disability and occupational impact of venom immunotherapy. BMJ Open. 2014;4:005593.Google Scholar
- Muller UR. Bee venom allergy in beekeepers and their family members. Curr Opin Allergy Clin Immunol. 2005;5:343–7.View ArticlePubMedGoogle Scholar
- Perez-Pimiento A, Prieto-Lastra L, Rodriguez-Cabreros M, Reano-Martos M, Garcia-Cubero A, Garcia-Loria J. Work-related anaphylaxis to wasp sting. Occup Med. 2007;57:602–4.View ArticleGoogle Scholar
- Bonadonna P, Schiappoli M, Dama A, Olivieri M, Perbellini L, Senna G, Passalacqua G. Is hymenoptera venom allergy an occupational disease? Occup Environm Med. 2008;65:217–8.View ArticleGoogle Scholar
- Siracusa A, Folletti I, van Gerth Wijk R, Jeebay MF, Moscato G, Quirce S, et al. Occupational anaphylaxis—an EAACI task force consensus statement. Allergy. 2015;70:141–52.View ArticlePubMedGoogle Scholar
- Shimizu T, Hori T, Tokuyama K, Morikawa A, Kuroume T. clinical and immunologic surveys of Hymenoptera hypersensitivity in Japanese forestry workers. Ann Allergy Asthma Immunol. 1995;74:495–500.PubMedGoogle Scholar
- Incorvaia C, Senna G, Mauro M, Bonadonna P, Marconi I, Asero E, Nitti F. Prevalence of allergic reactions to Hymenoptera stings in northern Italy. Eur Ann Allergy Clin Immunol. 2004;36:372–4.PubMedGoogle Scholar
- Copertaro A, Pucci S, Bracci M, Barbaresi M. Hymenoptera stings in forestry department agents: evaluation of risk. Med Lav. 2006;97:676–81.PubMedGoogle Scholar
- Mueller H. Diagnosis and treatment of insect sensitivity. J Asthma Res. 1966;3:331–3.View ArticlePubMedGoogle Scholar
- Muraro A, Roberts G, Worm M, Bilò MB, Brockow K, Fernandez Rivas M, et al. Anaphylaxis: guidelines from then european academy of allergy and clinical immunology. Allergy. 2014;69:1026–45.View ArticlePubMedGoogle Scholar
- Simons FE, Ardusso LR, Dimov V, Ebisawa M, El-Gamal YM, Lockey RF, et al. World Allergy Organization Anaphylaxis Giudelines: 2013 update of the evidence base. Int Arch Allergy Immunol. 2013;162:193–204.View ArticlePubMedGoogle Scholar
- Hayashih Y, Hirata H, Watanabe M, Yoshida N, Yojoyama T, Murayama Y, et al. Epidemiologic investigation of Hornet and Paper Wasp Stings in forest workers and electrical facility field workers in Japan. Allergol Int. 2014;63:21–6.View ArticlePubMedGoogle Scholar
- Pucci S, Antonicelli L, Bilò MB, Garritani MS, Bonifazi F. Allergy. 1994;49:894–6.View ArticlePubMedGoogle Scholar
- Viola F, Liuzzo L, Noto LV, Lo Conti F, La Loggia G. Spatial distribution of temperature trends in Sicily. Int J Climatol. 2014;34:1–17.View ArticleGoogle Scholar
- Demain JG, Gessner B, McLaughlin J, Sikes D, Foote J. Increasing insect reactions in Alaska:is this related to climate change? Allergy Asthma Proc. 2009;30:238–43.View ArticlePubMedGoogle Scholar
- Pucci S, D’Alò S, De Pasquale T, Illuminati I, Makri E, Incorvaia C. Risk of anaphylaxis in patients with large local reactions to hymenoptera stings: a retrospective and prospective study. Clin Mol Allergy. 2015;9(13):21.View ArticleGoogle Scholar
- Annila IT, Karjalainen ES, Annila PA, Kuusisto PA. Bee and wasp sting reactions in current beekeepers. Ann Allergy Asthma Immunol. 1996;77:423–7.View ArticlePubMedGoogle Scholar
- Fernandez J, Blanca M, Soriano V, Sanchez J, Juarez C. Epidemiological study of the prevalence of allergic reactions to Hymenoptera in a rural population in the Mediterranean area. Clin Exp Allergy. 1999;29:1069–74.View ArticlePubMedGoogle Scholar
- Kahan E, Ben-Moshe R, Derazne E, Tamir R. The impact of Hymenoptera venom allergy on occupational activities. Occup Med. 1997;47:273–6.View ArticleGoogle Scholar
- Ridolo E, Montagni M, Bonzano L, Savi E, Peveri S, Costantino MT, et al. How far from correct is the use of adrenaline auto-injectors? A survey in Italian patients. Intern Emerg Med. 2015;10:937–41.View ArticlePubMedGoogle Scholar