Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets
© Chirumbolo et al; licensee BioMed Central Ltd. 2010
Received: 18 May 2010
Accepted: 17 September 2010
Published: 17 September 2010
Flavonoids, a large group of polyphenolic metabolites derived from plants have received a great deal of attention over the last several decades for their properties in inflammation and allergy. Quercetin, the most abundant of plant flavonoids, exerts a modulatory action at nanomolar concentrations on human basophils. As this mechanism needs to be elucidated, in this study we focused the possible signal transduction pathways which may be affected by this compound. Methods: K2-EDTA derived leukocyte buffy coats enriched in basophil granulocytes were treated with different concentrations of quercetin and triggered with anti-IgE, fMLP, the calcium ionophore A23187 and the phorbol ester PMA in different experimental conditions. Basophils were captured in a flow cytometry analysis as CD123bright/HLADRnon expressing cells and fluorescence values of the activation markers CD63-FITC or CD203c-PE were used to produce dose response curves. The same population was assayed for histamine release.
Quercetin inhibited the expression of CD63 and CD203c and the histamine release in basophils activated with anti-IgE or with the ionophore: the IC50 in the anti-IgE model was higher than in the ionophore model and the effects were more pronounced for CD63 than for CD203c. Nanomolar concentrations of quercetin were able to prime both markers expression and histamine release in the fMLP activation model while no effect of quercetin was observed when basophils were activated with PMA. The specific phosphoinositide-3 kinase (PI3K) inhibitor wortmannin exhibited the same behavior of quercetin in anti-IgE and fMLP activation, thus suggesting a role for PI3K involvement in the priming mechanism.
These results rule out a possible role of protein kinase C in the complex response of basophil to quercetin, while indirectly suggest PI3K as the major intracellular target of this compound also in human basophils.
Flavonoids include a large group of low molecular weight polyphenolic secondary plant metabolites which can be found in fruits and vegetables, and plant derived beverages such as tea, wine and coffee [1–3]. More recently, these natural compounds have been recognized to exert antioxidant , anti-bacterial and anti-viral activity, in addition to anti-allergic effects [5–7], and exert anti-inflammatory , anti-angiogenic, analgesic, cardiovascular-protective , anti-hypertensive , hepatoprotective , cytostatic, cancer preventive , apoptotic , estrogenic and even anti-estrogenic properties . Quercetin (2-(3,4- dihydroxyphenyl)- 3,5,7- trihydroxy- 4H- chromen- 4-one) is the most abundant of the flavonoids and is commonly used as a food supplement , but evidence-based data regarding its clinical efficacy are quite scanty . As well as many other flavonols, quercetin exerts many effects on inflammation and allergic responses. In this context quercetin is known mainly as a strong inhibitor of many effector functions of leukocytes and mast cells at the micromolar concentration range: the flavonoid is able to inhibit histamine release from human basophils activated with different agonists [17–19], to decrease the expression of the basophil activation markers tetraspan CD63 and ectoenzyme CD203c , to block mast cell degranulation in the rat cell line RBL-2H3 model , to inhibit the production of pro-inflammatory cytokines in HMC-1 mast cell line . This evidence has led to the suggestion that quercetin might be a good candidate for immuno-modulation and anti-allergic therapy . Moreover, recent evidence from our laboratory has reported that sub-micromolar concentrations of quercetin, while inhibiting basophil activation marker expression in cells stimulated through an IgE-dependent pathway, are able to prime those markers in a classical non IgE-dependent activation pattern, such as using a formylated peptide (fMLP) as soluble agonist . The bimodal pattern showed by quercetin in basophils activated with fMLP, having the typical features of a classical hormetic mechanism [24, 25], prompted further investigation.
Aiming at understanding the bimodal mechanism by which quercetin acts on human basophils, in this study we investigated some basic signaling events in basophil activation, such as calcium, protein kinase C (PKC) and PI3K. From a molecular point of view, quercetin has a significant bulk of intracellular targets, mainly serine/threonine and tyrosine kinases, which is very difficult to disentangle . In basophil biology a first step to be forwarded could be investigating the differential pattern between anaphylactic degranulation and piecemeal degranulation, known to be related to IgE-mediated and to non-IgE mediated activation pathways, respectively and to the differential expression of surface molecules associated to cell activation . This issue can be focused by the use of inhibitors and regulatory molecules able to dissect these mechanisms. We used a polychromatic flow cytometry approach  to investigate the effect of the flavonoid quercetin on the expression of membrane markers triggered by several different agonists in normal subjects (healthy screened blood donors). In parallel, we also evaluated whether the effects of quercetin on basophil membrane markers were reproduced using a classical assay of histamine release. The huge collection of quercetin effects on countless cellular kinases, transcription factors and regulatory proteins, claims for further investigation about the molecular nature of its pharmacological action. This study, in addition to representing a contribute to the comprehension of basophil biology, gives new clues about the modulatory role of this natural compound in cells of inflammation and allergy.
N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), quercetin dihydrate (minimum 98% HPLC), phorbol-12-myristate-13-acetate (PMA), the ionophore A23187, the PI3K inhibitor wortmannin, Na3- ethylendiaminetetraacetic acid (EDTA), sodium heparin, trypan blue and distilled water (HPLC grade, Chromasolv® Plus) were all purchased from Sigma (Sigma-Aldrich GmbH, Germany). Goat anti-human IgE was purchased from Invitrogen-Caltag Laboratories, (UK). Histamine enzyme-linked immunosorbent assay (ELISA) releasing test was purchased from Labor Diagnostika Nord GmbH & Co., Germany. Mouse anti-human monoclonal antibodies for flow cytometry evaluation CD123-PECy5 (isotype IgG1, clone 6H6), CD45-APCCy7 (isotype IgG1 clone HI30), CD203c-PE (isotype IgG1 clone NP4D6), CD63-FITC (isotype IgG1 clone MEM-259) were purchased from Biolegend, San Diego CA, USA. HLA-DR-PECy7 (isotype IgG2a clone L243) was purchased from Becton Dickinson, Pharmigen CA, USA. Pure quercetin was dissolved in DMSO at a stock solution of 1 mg/ml and stored at +4°C for a maximum of 6 days; wortmannin was dissolved in DMSO at the stock concentration of 2 × 10-3 M, stored at -20°C and thawed before use. Working solutions were made into HEPES modified buffer (20 mM HEPES, 127 mM NaCl, 5 mM KCl, 5 UI/ml sodium-heparin, pH 7.4) (HBE). fMLP was dissolved in dimethylsulfoxide (DMSO) as a 2 × 10-2 M stock solution, stored at -20°C and thawed before use. The calcium ionophore A23187 and PMA were both dissolved in DMSO as stock solutions of 1 mg/ml (1.91 mM and 1.62 mM respectively), stored at -20°C and thawed before use. Working solutions of fMLP, anti-IgE, A23187 and PMA were freshly prepared in HBE supplemented with 5 mM CaCl2 and 2 mM MgCl2 (HBC buffer). All reagents were pure and quality checked; whenever necessary disposable plastic ware and sterile apyrogenic solutions were used.
Subjects and sampling
A total of 70 blood donors volunteers (47% male, 53% female) were enrolled in this study. Recruitment was randomized and encompassed an age range from 24 to 65 yrs (mean 44.61 ± 4.57 SD) in order to have a wide experimental population and to prevent age influence on cell releasability . All the subjects recruited in the study were non allergic and non atopic, they did not suffer of any immunological disorder and had never reported any previous history or genetic diathesis of chronic allergy; moreover, none underwent neither drug therapy nor anti-histamine therapy during the 48 hrs before the peripheral venous blood withdrawal. All participants completed and signed a specific consenting form for taking the samples and for data processing.
Cell recovery and preparation
Basophils were collected as leukocyte-enriched buffy coats from venous K2-EDTA anticoagulated peripheral blood from four screened healthy donors in each experiment performed, according to previously described methods . Buffy coats were pooled and suspended in HBE buffer. To count basophils and evaluate yields, an aliquot of about 1 ml of the cell culture was transferred to a Bayer ADVIA 2120 automated hematocytometer . The volume of working cell suspensions was adjusted with HBE buffer in order to get a basophil count of 90-150 basophils/μl. Compared with hemocytometer counts of starting whole blood (30-50 basophils/μl), an average enrichment of about 1.5-3.0 times (mean = 2.4) was currently obtained. Trypan blue exclusion test revealed that 98.7% ± 7.4 SD leukocytes were viable. Aliquots (100 μl) of cell samples were incubated at 37°C for 10 minutes with an equal volume of HBE in the absence or in the presence of quercetin or wortmannin at the indicated final doses. Activation was performed by adding 50 μl of treated cells to 50 μl of HBC buffer containing 200 nM fMLP or 8 μg/ml of goat anti-human IgE or 1.0 μM A23187 or 100 nM PMA, according to the different protocols. Resting assays were performed by incubating cells in HBC buffer without agonists. Incubation was carried out at 37°C for 30 minutes and blocked by adding 100 μl of ice-cold HBE supplemented with 2.8 mM sodium-EDTA (Na3-EDTA). Then the samples were put on ice and stained with monoclonal antibodies (20 minutes at +4°C), according to previously published methods . Afterwards, red blood cells underwent lysis with an ammonium-buffered solution (155 mM NH4Cl, 10 mM Na2HCO3, 0.10 mM Na3EDTA, pH = 7.2) for 4 minutes at +4°C; then samples were centrifuged at 700 g and pellets recovered and re-suspended in a PBS-buffered saline solution (pH 7.4) for flow cytometry reading.
Cells treated with different concentration of quercetin and activated with the indicated agonists, were pelleted at 6000 rpm for 5 minutes and surnatants collected for a competitive histamine ELISA test. 25 μl of each sample was treated with buffers and an acylation reagent, incubated for 1 hour at r.t. and diluted with 200 μl of distilled water. Aliquots of 20 μl of these acylated samples were incubated overnight with 100 μl of antiserum, washed 3 times, incubated for 1 hr at r.t. with a horseradish peroxidase-conjugate, washed 3 times with washing buffer, incubated for 30 min at r.t. with the colorimetric substrate tetramethylbenzidine and reaction stopped. The absorbance of the solution in each wells was read within 10 minutes at 450 nm with a reference wavelength of 620 nm. Histamine was calculated as ng/ml of the released amine against the corresponding standard concentrations in the calibration curve.
Flow cytometry and data processing
Basophil membrane markers were evaluated by flow cytometry using a five-color fluorochrome panel including CD45-APCCy7, CD123-PECy5 and HLA-DR-PECy7 as phenotyping markers and CD63-FITC and CD203c-PE as activation ones . Flow analysis was performed using a 488 nm-633 nm two-laser BD FACScanto flow cytometer: the instrument had a 10,000 events/sec capability, six-color detection and 0.1% sample carryover. Analysis were performed with a mean flow rate of 300-500 events/sec, setting an excess limit of 50,000 events to record in the basophil gate in order to analyze the whole buffered suspension volume and having a proper estimation of cell recovery and reproducibility. Compensation followed cytometer manufacturer's instruction according an off-line procedure by applying automated electronics algorithms and preset templates, by using biparametric logarithmic dot plots, gate-specific tubes and single-tube data analysis, and optimizing FSC threshold and fluorochrome voltage as set up parameters. Mean of fluorescence intensity (MFI) was calculated automatically by the cytometer software. Percentage of activated cells was calculated by the software considering the CD63 expressing cells (CD63-FITCpositive cells) counted to the right of a threshold that was established including the main peak of fluorescence of a sample of resting cells. In order to reduce standard deviation due to positive fluorescent cells respect to negative or dimly ones, a logarithmic scale and a coefficient of variation to measure variability dispersion were used.
Data were analyzed using the software SPSS, version 11 for Windows, Chicago, IL. Dose response curves were obtained by plotting the triplicate data and their mean values and S.E.M. for each experiment using the Sigma plot 10 software. Kolmogorov-Smirnov and Shapiro-Wilk goodness-of-fit tests were performed to determine whether the sample population followed a Gaussian distribution. Differences between quercetin-treated and non-treated cells were analyzed by using a one-way analysis of variance (ANOVA) followed by Fisher LSD test. A value of p < 0.05 was considered statistically significant. IC50 was calculated for each curve of percentage of effect/control by a linear regression calculation according to the four parameter logistic model (4PL), also called the Hill-Slope model.
Basophils stimulated with anti-IgE or fMLP
Basophils stimulated with the calcium ionophore A23187 or with PMA
Basophil releasability by the histamine ELISA test
Q1) Effects of quercetin in the FcεRI-anti-IgE activation model. Previously published reports have shown that quercetin is able to inhibit PI3K by binding to the catalytic pocket of the enzyme: as for instance, LY294002, a synthetic inhibitor of PI3K, has actually a chemical kinship with the flavonoid quercetin . The IC50 for quercetin as an inhibitor of PI3K from human blood platelets is around 1.8-20 μM , which corresponds to the inhibitory range observed in the results here reported on basophils. Taking into account the downstream signaling pathway of FcεRI-anti-IgE complex, a suggestion would come that the inhibition of PI3K leads to the loss of phosphorylation of downstream kinases such as Bruton's tyrosine kinase (BTK)  which in turn is able to phosphorylate PLCγ, thus leading to the production of inositol-1,4,5-triphosphate (IP3) and to diacylglycerol (DAG) from the precursor phosphatidylinosytol-4,5-biphosphate (PtdIns 4,5-P2 or PIP2) (Figure 7). While DAG remains to the membrane, IP3 diffuses to the cytosol and binds to and activates the InsP3 receptor on the membrane of the endoplasmic reticulum, opening a calcium channel, resulting in the release of Ca2+ into the cytoplasm. DAG is able to activate PKC, which in turn activates membrane markers up-regulation and histamine release . Warner et al. observed that the amount of histamine release associated with activation of basophils through IgE receptor aggregation, among different preparations of basophils, was correlated with an increase in membrane bound PKC-like activity . These results also suggested that PKC activation may have a role in IgE-mediated histamine release in human basophils and that quercetin might inhibit basophil function by blocking DAG precursor for PKC in the upstream signaling pathways. The inhibition of PI3K by quercetin would also prevent the formation of phosphatidylinositol 3,4,5-triphosphate (PtdIns3,4,5-P3) which activates extracellular calcium influx by membrane Ca++ channels .
Q2) Effects of quercetin in the FPR-fMLP activation model. Figure 7 depicts a speculative suggestion concerning the priming phenomenon observed on the fMLP-triggered basophil function. Since in our assay system the effects of quercetin were superimposable to those of wortmannin, a potent PI3K inhibitor [42, 36], our results indirectly suggest a role for PI3K in the dual effects performed by the flavonoid. G-protein coupled receptors, such as the fMLP receptor, activate the PI3Kγ isoform through interactions with Gβγ of the PI3K p101 and p110γ subunits . Increasing evidence suggests that monomeric p110γ may function as a downstream regulator of G-protein coupled receptor dependent signal transduction : Gβγ is able to activate a G-coupled receptor kinase (GRK) which desentitizes the receptor. Interactions of quercetin with this Gβγ-p101/p110γ might exert an action leading to these possible results: a) the inability of Gβγ sequestered by p101/p110γ complex to activate G-coupled receptors kinases (GRKs) and to desensitize the receptor, leading to a priming mechanism for example by inducing a sustained activation of downstream protein kinases involved in the degranulatory event, such as p38-MAPK ; b) the long-lasting activation of Gβγ-associated PLCβ due to a defect in the Gβγ/PI3K dissociation, leading to an increase in signaling mediators able to trigger the degranulation event (by IP3-calcium signaling or by the activation of DAG-PKC pathway), so resulting in a priming effect.
Q3) Effect of quercetin on protein kinase C (PKC) activation pathway. In our assay system the flavonol proved insensitive to target protein kinase C (PKC), as resulted from the use of PMA as basophil stimulant, thus confirming previous reports : quercetin was unable to inhibit CD63 and CD203c membrane up-regulation in basophils stimulated with phorbol esters and no dissociation between the two markers investigated was actually observed by using PMA . So, the PKC pathway triggered by PMA, and presumably by other physiologic stimulants, is a quercetin-insensitive route to basophil activation.
Q4) Effect of quercetin on basophils triggered with calcium ionophore A23187. In response to calcium ionophore A23187 the expression of both the activation markers CD63 and CD203c was markedly up-regulated, but quercetin exerted a significant inhibitory action, even at nanomolar doses, only on CD63, so dissecting the response of the two activation markers to the ionophore. Previous evidence has reported that CD203c and CD63 upregulation in response to calcium signal by A23187 showed different kinetics , an evidence that probably suggests different pathways of calcium involvement in the expression of the two markers . Our results indicate that the calcium-mediated signaling is essential both for the LAMP-1 CD63 and for the ENPP-3 CD203c upregulation, as A23187-mediated calcium influx stimulate both the expression of basophil activation markers and histamine release (see Figure 7), but on the same time they suggest also that the transduction pathway diverges in two distal branches, one of which (LAMP-1) is sensitive to quercetin and is related to the degranulatory event , the other is much more resistant to this inhibition. It is well known that A23187 promotes the activation of Ca++/calmodulin pathway , which is inhibited by quercetin . Calmodulin constitutes an obligate link in signal transduction pathways leading to human leukocyte histamine release if the trigger is a calcium ionophore but not when responses are induced by anti-IgE, fMLP or PMA . Quercetin ability to target calmodulin drives to the suggestion that those events inhibited by the flavonoid, i.e. the histamine release and CD63 membrane up-regulation, were presumably related to a Ca++/calmodulin dependent pathway in basophils activated with A23187, while the expression of CD203c, which was not significantly affected by the flavonoid even at its highest dose, might be a calmodulin-independent event. This marker is probably translocated to the membrane by other calcium dependent vesicular-transport mechanisms .
These hypotheses and models need for further investigation on a molecular level such as a direct demonstration of the kinases isolated from or detected in the purified basophils and/or by using isoform-selective inhibitors of PI3K and to assay calmodulin involvement in the A23187 activation pathway inhibited by quercetin. What is really interesting is that the observed modulatory biphasic (hormetic) mechanism can be related to the inhibition of PI3K by quercetin and that the efficacious doses are within the nanomolar plasma concentrations reported in several pharmacokinetic and bioavailability studies about this flavonoid [51–53]. At these concentrations is commendable that quercetin exerts a fine regulatory action depending on the fine balancing of signaling proteins ruled by PI3Ks. The PI3Ks seem to be strategic both for the activation of downstream protein kinases and for receptor-associate phospholypases C activation thus leading to calcium elevation in the cytoplasm and to PKC-mediated degranulation, two conditions which basophil needs for up-loading its markers of activation on the membrane and for histamine release. This might be a first step by which quercetin is able to exert its action at sub-micromolar-nanomolar concentration range, while at the highest doses its action might involve also other receptor and PI3K-downstream kinases such as Akt/PKB, MEK, p38-MAPK, etc. .
Allergy is a cause for concern, mainly due to its rising prevalence inside the population and to the increasing difficulty in treating chronic allergy. Quercetin might be a good candidate with the potential to counter this trend: an appropriate intake of this flavonol from food and beverage or from supplemental administration could be expected to improve allergy, to help anti-inflammatory and anti-oxidative responses by the organism and to prevent the onset of allergic chronic diseases. However our results introduce a caveat: although basophils play an important role in mediating allergic response and quercetin has proved to have an inhibitory action on basophils following stimulation with anti-IgE and calcium ionophore A23187, the existing bimodal effects of the flavonol and the complex nature of hypersensitivity reactions would require researchers be more cautious before considering quercetin in the practical use of the therapy and prevention of allergy. To achieve this goal, further research insights about cell signaling and about quercetin intracellular targets and studies in animal models are required.
List of abbreviations
Enzyme-linked immunosorbent assay
ectonucleotide pyrophosphatase phosphodiesterase-3
G-coupled receptor kinase
human mast cell line-1
lysosome associated membrane protein-3
Mean of fluorescence intensity
protein kinase C
p38-mitogen activated protein kinase
rat basophilic leukemia cell line.
This work is supported by grants from Ministero dell'Università e della Ricerca.
- Hertog MG, Hollman PC: Potential health effects of the dietary flavonol quercetin. Eur J Clin Nutr. 1996, 50: 63-71.PubMed
- Hollman PC, Katan MB: Dietary flavonoids: intake, health effects and bioavailability. Food Chem Toxicol. 1999, 37: 937-942. 10.1016/S0278-6915(99)00079-4View ArticlePubMed
- Hollman PC, Katan MB: Health effects and bioavailability of dietary flavonols. Free Radic Res. 1999, 31 (Suppl): S75-S80. 10.1080/10715769900301351View ArticlePubMed
- Woodman OL, Chan EC: Vascular and anti-oxidant actions of flavonols and flavones. Clin Exp Pharmacol Physiol. 2004, 31: 786-790. 10.1111/j.1440-1681.2004.04072.xView ArticlePubMed
- Kawai M, Hirano T, Higa S, Arimitsu J, Maruta M, Kuwahara Y, Ohkawara T, Hagihara K, Yamadori T, Shima Y, Ogata A, Kawase I, Tanaka T: Flavonoids and related compounds as anti-allergic substances. Allergol Int. 2007, 56: 113-123. 10.2332/allergolint.R-06-135View ArticlePubMed
- Jeong KW, Lee JY, Kang DI, Lee JU, Shin SY, Kim Y: Screening of flavonoids as candidate antibiotics against Enterococcus faecalis. J Nat Prod. 2009, 72: 719-724. 10.1021/np800698dView ArticlePubMed
- Cushnie TP, Lamb AJ: Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005, 26: 343-356. 10.1016/j.ijantimicag.2005.09.002View ArticlePubMed
- Garcia-Lafuente A, Guillamon E, Villares A, Rostagno MA, Martinez JA: Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflamm Res. 2009, 58 (9): 537-52. 10.1007/s00011-009-0037-3View ArticlePubMed
- Terao J, Kawai Y, Murota K: Vegetable flavonoids and cardiovascular disease. Asia Pac J Clin Nutr. 2008, 17 (Suppl 1): 291-293.PubMed
- Perez-Vizcaino F, Duarte J, Jimenez R, Santos-Buelga C, Osuna A: Antihypertensive effects of the flavonoid quercetin. Pharmacol Rep. 2009, 61: 67-75.View ArticlePubMed
- Jayaraj R, Deb U, Bhaskar AS, Prasad GB, Rao PV: Hepatoprotective efficacy of certain flavonoids against microcystin induced toxicity in mice. Environ Toxicol. 2007, 22: 472-479. 10.1002/tox.20283View ArticlePubMed
- Wang L, Lee IM, Zhang SM, Blumberg JB, Buring JE, Sesso HD: Dietary intake of selected flavonols, flavones, and flavonoid-rich foods and risk of cancer in middle-aged and older women. Am J Clin Nutr. 2009, 89: 905-912. 10.3945/ajcn.2008.26913PubMed CentralView ArticlePubMed
- Galluzzo P, Martini C, Bulzomi P, Leone S, Bolli A, Pallottini V, Marino M: Quercetin-induced apoptotic cascade in cancer cells: antioxidant versus estrogen receptor alpha-dependent mechanisms. Mol Nutr Food Res. 2009, 53: 699-708. 10.1002/mnfr.200800239View ArticlePubMed
- Zava DT, Duwe G: Estrogenic and antiproliferative properties of genistein and other flavonoids in human breast cancer cells in vitro. Nutr Cancer. 1997, 27: 31-40. 10.1080/01635589709514498View ArticlePubMed
- Boots AW, Haenen GR, Bast A: Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol. 2008, 585: 325-337. 10.1016/j.ejphar.2008.03.008View ArticlePubMed
- Bischoff SC: Quercetin: potentials in the prevention and therapy of disease. Curr Opin Clin Nutr Metab Care. 2008, 11: 733-740. 10.1097/MCO.0b013e32831394b8View ArticlePubMed
- Middleton E Jr, Drzewiecki G, Krishnarao D: Quercetin: an inhibitor of antigen-induced human basophil histamine release. J Immunol. 1981, 127: 546-550.PubMed
- Middleton E Jr, Krishnarao DG, Atkins D, Drzewiecki G: The flavonoids: a brief review and study of effects on antigen-induced histamine release from human basophils. Trans Am Clin Climatol Assoc. 1981, 92: 234-252.PubMed CentralPubMed
- Middleton E Jr, Drzewiecki G: Flavonoid inhibition of human basophil histamine release stimulated by various agents. Biochem Pharmacol. 1984, 33: 3333-3338. 10.1016/0006-2952(84)90102-3View ArticlePubMed
- Chirumbolo S, Conforti A, Ortolani R, Vella A, Marzotto M, Bellavite P: Stimulus-specific regulation of CD63 and CD203c membrane expression in human basophils by the flavonoid quercetin. Int Immunopharmacol. 2010, 10: 183-192. 10.1016/j.intimp.2009.10.014View ArticlePubMed
- Passante E, Ehrhardt C, Sheridan H, Frankish N: RBL-2H3 cells are an imprecise model for mast cell mediator release. Inflamm Res. 2009, 58: 611-618. 10.1007/s00011-009-0028-4View ArticlePubMed
- Park HH, Lee S, Son HY, Park SB, Kim MS, Choi EJ, Singh TS, Ha JH, Lee MG, Kim JE, Hyun MC, Kwon TK, Kim YH, Kim SH: Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. Arch Pharm Res. 2008, 31: 1303-1311. 10.1007/s12272-001-2110-5View ArticlePubMed
- Shaik YB, Castellani ML, Perrella A, Conti F, Salini V, Tete S, Madhappan B, Vecchiet J, De Lutiis MA, Caraffa A, Cerulli G: Role of quercetin (a natural herbal compound) in allergy and inflammation. J Biol Regul Homeost Agents. 2006, 20: 47-52.PubMed
- Calabrese EJ, Baldwin LA: Hormesis as a biological hypothesis. Environm Health Perspect. 1998, 106: 357-362. 10.2307/3433938.View Article
- Calabrese EJ: Hormesis: principles and applications for pharmacology and toxicology. Am J Pharmacol Toxicol. 2008, 3: 59-71. 10.3844/ajptsp.2008.59.71.View Article
- Williams RJ, Spencer JP, Rice-Evans C: Flavonoids: antioxidants or signalling molecules?. Free Radic Biol Med. 2004, 36: 838-849. 10.1016/j.freeradbiomed.2004.01.001View ArticlePubMed
- Dvorak AM: Ultrastructural studies of human basophils and mast cells. J Histochem Cytochem. 2005, 53: 1043-1070. 10.1369/jhc.5R6647.2005View ArticlePubMed
- Chirumbolo S, Vella A, Ortolani R, De Gironcoli M, Solero P, Tridente G, Bellavite P: Differential response of human basophil activation markers: a multi-parameter flow cytometry approach. Clin Mol Allergy. 2008, 6: 12. 10.1186/1476-7961-6-12PubMed CentralView ArticlePubMed
- Marone G, Poto S, Columbo M, Quattrin S, Condorelli M: Histamine release from human basophils in vitro: effects of age of cell donor. Monogr Allergy. 1983, 18: 139-143.PubMed
- Harris N, Jou JM, Devoto G, Lotz J, Pappas J, Wranovics D, Wilkinson M, Fletcher SR, Kratz A: Performance evaluation of the ADVIA 2120 hematology analyzer: an international multicenter clinical trial. Lab Hematol. 2005, 11: 62-70. 10.1532/LH96.04064View ArticlePubMed
- Middleton E Jr, Drzewiecki G, Krishnarao D: Quercetin: an inhibitor of antigen-induced human basophil histamine release. J Immunol. 1981, 127: 546-550.PubMed
- Middleton E Jr: Effect of flavonoids on basophil histamine release and other secretory systems. Prog Clin Biol Res. 1986, 213: 493-506.PubMed
- Egert S, Wolffram S, Bosy-Westphal A, Boesch-Saadatmandi C, Wagner AE, Frank J, Rimbach G, Mueller MJ: Daily quercetin supplementation dose-dependently increases plasma quercetin concentrations in healthy humans. J Nutr. 2008, 138: 1615-1621.PubMed
- Moon JH, Nakata R, Oshima S, Inakuma T, Terao J: Accumulation of quercetin conjugates in blood plasma after the short-term ingestion of onion by women. Am J Physiol Regul Integr Comp Physiol. 2000, 279: R461-R467.PubMed
- Conquer JA, Maiani G, Azzini E, Raguzzini A, Holub BJ: Supplementation with quercetin markedly increases plasma quercetin concentration without effect on selected risk factors for heart disease in healthy subjects. J Nutr. 1998, 128: 593-597.PubMed
- Walker EH, Pacold ME, Perisic O, Stephens L, Hawkins PT, Wymann MP, Williams RL: Structural Determinants of Phosphoinositide 3-Kinase Inhibition by Wortmannin, LY294002, Quercetin, Myricetin, and Staurosporine. Mol Cell. 2000, 6: 909-919. 10.1016/S1097-2765(05)00089-4View ArticlePubMed
- Agullo G, Gamet-Payrastre L, Manenti S, Viala C, Remesy C, Chap H, Payrastre B: Relationship between flavonoid structure and inhibition of phosphatidylinositol 3-kinase: a comparison with tyrosine kinase and protein kinase C inhibition. Biochem Pharmacol. 1997, 53: 1649-1657. 10.1016/S0006-2952(97)82453-7View ArticlePubMed
- Rommel C, Camps M, Ji H: PI3Kd and PI3Kg: partners in crime in inflammation in rheumatoid arthritis and beyond?. Nature Rev Immunol. 2007, 7: 191-201. 10.1038/nri2036.View Article
- Miura K, Macglashan DW Jr: Expression of protein kinase C isozymes in human basophils: regulation by physiological and nonphysiological stimuli. Blood. 1998, 92: 1206-1218.PubMed
- Warner JA, Macglashan DW Jr: Protein kinase C (PKC) changes in human basophils. IgE-mediated activation is accompanied by an increase in total PKC activity. J Immunol. 1989, 142: 1669-1677.PubMed
- Viard P, Butcher AJ, Halet G, Davies A, Nurnberg B, Heblich F, Doplhin AC: PI3K promotes voltage-dependent calcium channel trafficking to the plasma membrane. Nature Neuroscience. 2004, 7: 939-946. 10.1038/nn1300View ArticlePubMed
- Arcaro A, Wymann MP: Wortmannin is a potent phosphoinositide-3 kinase inhibitor: the role of phosphatidylinositol-3, 4, 5 triphosphate in neutrophil responses. Biochem J. 1993, 296: 297-301.PubMed CentralView ArticlePubMed
- Brock C, Schaefer M, Reusch HP, Czupalla C, Michalke M, Spicher K, Schultz G, Nurnberg B: Roles of Gbetagamma in membrane recruitment and activation of p110gamma/p101 phosphoinositide 3-kinase gamma. J Cell Biol. 2003, 160: 89-99. 10.1083/jcb.200210115PubMed CentralView ArticlePubMed
- Mocsai A, Jakus Z, Vantus T, Berton G, Lowell CA, Ligeti E: Kinase pathways in chemoattractant-induced degranulation of neutrophils: the role of p38 mitogen-activated protein kinase activated by Src family kinases. J Immunol. 2000, 164: 4321-4331.View ArticlePubMed
- Hennersdorf F, Florian S, Jakob A, Baumgartner K, Sonneck K, Nordheim A, Biedermann T, Valent P, Buhring HJ: Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of ige-dependent upregulation. Cell Res. 2005, 15: 325-335. 10.1038/sj.cr.7290301View ArticlePubMed
- Woo CS, Chan W, Sampson HA, Shreffler WG: Cell-by-cell characterization of signaling and activation marker upregulation during basophil stimulation and non specific desensitization. J All Clin Immunol. 2009, 123: S199-post 764.
- de Weck AL, Sanz ML, Gamboa PM, Aberer W, Bienvenu J, Bianca M, Demoly P, Ebo DG, Mayorga L, Monneret G, Sainte-Laudy J: Diagnostic tests based on human basophils: more potentials and perspectives than pitfalls: II Technical issues. J Invest Allergol Clin Immunol. 2008, 18: 143-155.
- Bergstrand H, Lundquist B: Human basophil histamine release is differently affected by inhibitors of calmodulin, diacylglycerol kinase and peptidyl prolyl cis-trans isomerase in a secretagogue specific manner. Allergy. 2007, 47: 353-361. 10.1111/j.1398-9995.1992.tb02071.x.View Article
- Nishino H, Naitoh E, Iwashima A, Umezawa K: Quercetin interacts with calmodulin, a calcium regulatory protein. Experientia. 1984, 40: 184-185. 10.1007/BF01963590View ArticlePubMed
- Dvorak AM: Ultrastructural studies of human basophils and mast cells. J Histochem Cytochem. 2005, 53: 1043-1070. 10.1369/jhc.5R6647.2005View ArticlePubMed
- Hollman PC, Katan MB: Bioavailability and health effects of dietary flavonols in man. Arch Toxicol Suppl. 1998, 20: 237-248.View ArticlePubMed
- Graefe EU, Derendorf H, Veit M: Pharmacokinetics and bioavailability of the flavonol quercetin in humans. Int J Clin Pharmacol Ther. 1999, 37: 219-233.PubMed
- Erlund I, Freese R, Marniemi J, Hakala P, Alfthan G: Bioavailability of quercetin from berries and the diet. Nutr Cancer. 2006, 54: 13-17. 10.1207/s15327914nc5401_3View ArticlePubMed
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