Fig. 4

Frequency of Tregs in spleen cells. Flow cytometry data showing the percentage of FoxP3⁺CD25⁺ cells on gated CD4⁺ cells of total spleen cells. The d2-OID treatment produced an increase of this subpopulation of regulatory cells (p < 0.05 vs sham) but not as high as for the other groups of treatment. Either VD₃ or d2-OID administration induced the highest increase in FoxP3⁺CD25⁺ cells, compared with sham (p < 0.01, for both groups) or d2-OID (p < 0.05, for both groups). Values are expressed as mean ± SD (n = 12). *p < 0.05 and **p < 0.01