IgE reactivity of patient sera. IgE reactivity of individual patient sera from double-positive (A) or YJV-positive patients (B) to rVes v 1 and rVes v 5 produced in Sf9 insect cells, nApi m 1, and MUXF-BSA. The cut-off of the ELISA is indicated by a dashed line (mean background plus two fold the SD). The percentage of sIgE reactivity to either each allergen (Ves v 1 only/Ves v 5 only) or both allergens (Ves v 1 and Ves v 5) is represented in C for double-positive (black bars) or YJV-positive (grey bars) patients. The overall sIgE reactivity to Ves v 1 or Ves v 5 (sum Ves v 1 or sum Ves v 5) was obtained by addition of exclusive sIgE reactivity to one allergen to sIgE reactivity if not recognized exclusively. Overall diagnostic sensitivity (sum) was obtained by addition of all sIgE reactivities.