Figure 5

Effects of BAI on NF-κB translocation in IL-1β- and TNF-α-activated HMC-1 cells. HMC-1 cells were cultured with IL-1β or TNF-α in the presence or absence of BAI (30 μM) for 24 hours. NF-κB translocation was analyzed by a shift in oligonucleotide binding in EMSA gels. NF-κB translocation was decreased by BAI co-cultured with IL-1β (panel A) and TNF-α (panel B) when compared with the IL-1β or TNF-α alone. Densitometric analysis of NF-κB was expressed as integrated intensity and shown in the bar graph.