Figure 3
Effects of Baicalein (BAI) on production of IL-6, IL-8, and MCP-1 from TNF-α-activated HMC-1 cells. To each well of a 6-well culture plate, two ml of HMC-1 (1 × 106 cells/ml) were cultured alone (Control), or in the presence of BAI (30 μM), TNF-α (100 U/ml), and the combinations of TNF-α (100 U/ml) with BAI (30 μM) for 24 hrs in triplicate. Supernatants were harvested for measuring IL-6, IL-8, and MCP-1 by ELISA. The IL-6(Panel A), IL-8 (Panel B), and MCP-1 (Panel C) production was significantly decreased when BAI was added in TNF-α-activated HMC-1. + and # indicate p <0.0005 and <0.00005, respectively, when compared with the TNF-α-treated group.