Th1/Th2 responses of the separated and cultured peripheral blood mononuclear cells (PBMCs) in response to specific antigen stimulation. IL-4 levels (A) represent Th2 response and IFN-γ levels (B) represent Th1 response. The IL-4 (C) and IFN-γ (D) positively stained cells were detected by immune staining and flow cytometry. Data are expressed as mean ± SD. *, p < 0.05, compared with no treatment cells.