Figure 1

SDS-PAGE and immunoblot analysis of Beauveria bassiana crude extracts. SDS-PAGE, Gelcode blue stained, lanes A) 5 μg protein standards, and B) 40 μg B. bassiana crude extract. Immunoblots probed with pooled serum mix-I (patients displaying mould allergies), lanes 1), 5 μg protein standards, 2) 20 μg B. bassiana crude extract, 3) 40 μg crude extract, 4) 40 μg crude extract, Proteinase K treated, 5) 40 μg crude extract, denaturing buffer control (no EndoH), 6) 40 μg crude extract, EndoH treated