Defining the molecular role of gp91phox in the immune manifestation of acute allergic asthma using a preclinical murine model

Table 2 Clonogenic potential of progenitors

	PB/2 ml	BM/2femurs	LP/2lungs	BAL/2lungs	Spleen
WA	5371.6 ± 188.6	17034.5 ± 158.8	241.93 ± 11.8	3.15 ± 2.13	75858.9 ± 523.5
WO	16915.6 ± 219.1	57161.5 ± 161.9	11225.4 ± 155	5573.8 ± 41.6	188275.6 ± 361.1
NOXA	2343 ± 244.3	8293.3 ± 197.1	604.1 ± 125.8	59.9 ± 19.1	41774.2 ± 75.4
NOXO	9403.3 ± 382.2	40677.7 ± 220.4	6299.3 ± 195.9	5921.7 ± 41.63	219677.5 ± 426.8
DKOA	4591.6 ± 256.6	11783.1 ± 284.5	562.9 ± 183.7	32.9 ± 45.7	65974.3 ± 344.3
DKOO	9262.1 ± 342.7	41278.3 ± 361.7	7103.1 ± 201.6	6012.2 ± 59.7	197822.7 ± 388.7

Decrease in clonogenic potential in bone marrow and lung of both KO mice post-OVA. CFU (Colony Forming Units) as assay was done as described in Materials and Methods and assayed in duplicate petridishes per sample. The results shown are pooled from three independent experiments (n = 5/group) ± SEM. Abbreviations used are: BM, bone marrow; PB, peripheral blood, LP, lung parenchyma, BALf, bronchoalveolar lavage fluid. The total number of progenitors was extrapolated to the total number cells obtained from each tissue. Underlined numbers denote p value < 0.01 compared to post-OVA WT.

ISSN: 1476-7961