Figure 5

Partial amino acid sequences derived by mass spectrometry for the sugar beet pollen allergens Beta v 1 and Beta v 2. Peptide sequence data for Beta v 1 (A) and Beta v 2 (B) was obtained, from samples excised from SDS-PAGE (Fig. 1) and 2DE (Fig. 4A), by aquisition of MS and MS/MS-data utilizing sequences of ten homologues for Che a 1 and Che a 2 respectively for matching and inclusion lists. Sequences matching peptide masses in MS-data are indicated in bold in the various homologous sequences. Peptides confirmed by MS/MS are underlined in the sequences for Beta v 1 and Beta v 2. Enboxed: IgE-binding epitope of profilin, overlapping with actin-binding site [27]. Multiple alignments were performed with Clustal-W http://www.ebi.ac.uk/.