Research Upregulation of CD23 (FcεRII) Expression in Human Airway Smooth Muscle Cells (huASMC) in Response to IL-4, GM-CSF, and IL-4/GM-CSF

Table 1 Il-4, GM-CSF, and IL-4/GM-CSF Increase CD23 Expression on ASMC (n = 3)

Cytokine (24 hours)	#Cells in Gate D (n = 3)	%Cells with Increased CD23 Expression Above the Control
BSA (1 μg/ml)	1,343 ± 122	0
IL-4 (0.5 nM)	1,413 ± 197	25.1 ± 4.2*
GM-CSF (0.4 nM)	1,346 ± 243	15.6 ± 2.7*
IL-4/GM-CSF (0.4/0.5 nM)	1,324 203	32.9 ± 13.9*
IL-5 (0.4 nM)	1,130 ± 251	0
IL-13 (0.4 nM)	1,316 ± 269	0
PGD2 (1 μM)	1,521 ± 123	0
PGD2 (10 μM)	1,159 ± 204	0
LTD4 (10 μM)	2,037 ± 375	0
Ethanol (6% vol/vol)	2,507 ± 200	0
Tryptase (10 μM)	2,385 ne ± 405	0

Alpha-smooth muscle isoactin positive Human ASMC (Clonetics) in T-75 flasks were starved for 24 h in 0.1% FBS containing medium M199. The cells were then stimulated with BSA (1 μg/ml), IL-4 (0.5 nM), GM-CSF (0.4 nM), IL-4 (0.5 nM)/GM-CSF (0.4 nM), IL-13 (0.4 nM), IL-5 (0.4 nM), PGD2 (10 μM), LTD4 (10 μM), tryptase (30 nM) for 24 h. Results are mean ± SEM of the percentage of cells with an increased fluorescence intensity above the control (n = 3). Control values: BSA-stimulated, PE-anti-CD23 labeled, 10.9 ± 1.4 %; BSA-stimulated, PE-mouse IgG₁, non-immune, 0.5 ± 0.1 % (n = 3). *denotes significant increase in CD23 expression above the BSA control value.

ISSN: 1476-7961