Figure 2

Upregulation of CD23 by IL-4, GM-CSF and IL-4/GM-CSF. Alpha-smooth muscle isoactin positive Human ASMC (Clonetics) in T-75 flasks were starved for 24 h in 0.1% FBS containing medium M199. The cells were then stimulated with IL-4 (0.5 nM)/GM-CSF (0.4 nM) for 24 h. The FACS analysis showed that the smaller cells passed through Gate A and larger cells passed through Gate D. Background noise was eliminated using the BSA-stimulated control cells that were labeled with PE-anti-CD23 (EBVCS), represented by C in Gate A, and F in Gate D. The FACS results of a representative experiment showed 66% of the larger cells (Gate D) had an increase in cell expression of CD23 when compared to the controls.