Skip to main content

Table 1 Basophil cell yields. Average of numbers detected after lysis at 7 different concentrations of CRA1 from 250000 (normalized) events after lysis with either WBL or Immunoprep/Q-prep (average ± SD, tested with a paired t test). + = atopics, - = non atopics

From: Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI

Donor

Allergy

WBL ± SD

Immunoprep ± SD

p-value

1

-

1714 ± 314

1108 ± 449

<0,015

2

-

1979 ± 132

369 ± 274

<0,001

3

+

1638 ± 66

406 ± 262

<0,001

4

-

568 ± 84

182 ± 107

<0.001

5

-

1646 ± 282

371 ± 248

<0,001

6

+

483 ± 113

436 ± 138

0,494

7

-

1164 ± 70

213 ± 160

<0.001

8

-

1132 ± 372

582 ± 150

0,003

9

-

1397 ± 207

306 ± 133

<0,001

10

-

995 ± 481

577 ± 415

0,107

11

-

407 ± 128

397 ± 183

0,904

Median

 

1164

397