Comparison of: | Assays IMMULITE ImmunoCAP | Type of study | Type of comparison |
---|---|---|---|
Whole extract | EW (n = 36) sIgE HDM (n = 44) sIgE | Correctness by sample dilution linearity | Ratio of observed value and expected value (O/E) |
Allergen-specific mouse-human chimeric IgE monoclonal antibodies | Der p 1, Der p 2 Gal d 1, Gal d 2, Gal d 4 sIgE Total IgE (ImmunoCAP) | Accuracy by sIgE/tIgE agreement in dilution series | Ratio of observed value and total IgE value (sIgE/tIgE) |
Mouse monoclonal ab (IgG) addition test with 3 concentrations: | Interference study | Ratio of observed value to the control (initial level with no addition of mIgG ab) | |
Anti-Der p 1 mIgG | HDM sIgE | ||
Anti-Gal d 2 mIgG | EW sIgE | ||
Addition of Der p 2-chimeric IgG4 antibody to 2 sIgE positive patient samples previously tested for house dust mite (13.4 kUA/L and 78.7 kUA/L) | HDM sIgE | Interference study | Ratio of observed value to the control (initial level with no addition of IgG4 ab) |