Fig. 2
From: Safety of measles, mumps, and rubella vaccine in egg allergy: in vivo and in vitro management
Ex vivo B-cell proliferation assay. Cell proliferation assessed by reduction of CFSE intensity: a in microcultures of untreated B-cells; b B-lymphocytes incubated with a 1:4000 MMR vaccine dilution; c B-lymphocytes incubated with a 1:400 MMR vaccine dilution; and, finally, d B-lymphocytes incubated with a 1:40 MMR vaccine dilution. Results refer to one of the 2 proliferation assays carried out. In e Stimulation Indexes for the 3 MMR vaccine dilutions above. Averages of the 2 assays. The Stimulation Index is the ratio between the proliferation rate of cells exposed to a potential proliferation agent and the basal proliferation rate of control cells. A Stimulation Index ≥ 2 indicates a significant specific proliferation activity. SSC side scatter, CFSE carboxyfluorescein succimydil ester