Fig. 5
Effects of r-Nef on CXCL8/IL-8 and CCL3/MIP-1α release from human basophils and mast cells. a 106 purified basophils/sample were incubated for 4 or 18 h without (white histogram) or with r-Nef (100 ng/ml) (grey histogram). Supernatants were collected at each time point. CXCL8/IL-8 was determined by ELISA. Values are the mean ± SEM of three distinct experiments. *p < 0.05 as compared to basophils preincubated in the absence of chemotactic stimuli. b 106 purified basophils/sample were incubated for 4 or 18 h without (white histogram) or with r-Nef (100 ng/ml) (grey histogram). Supernatants were collected at each time point. CCL3/MIP-1α was determined by ELISA. Values are the mean ± SEM of three distinct experiments. *p < 0.05 as compared to basophils preincubated in the absence of chemotactic stimuli. c 106 HLMC cells/sample were incubated for 12 or 24 h without (white histogram) or with r-Nef (100 ng/ml) (grey histogram). Supernatants were collected at each time point. CXCL8 was determined by ELISA. Values are the mean ± SEM of three distinct experiments. *p < 0.05 as compared to mast cells preincubated in the absence of chemotactic stimuli. d 106 HLMC cells/sample were incubated for 12 or 24 h without (white histogram) or with r-Nef (100 ng/ml) (grey histogram). Supernatants were collected at each time point. CCL3/MIP-1α was determined by ELISA. Values are the mean ± SEM of three distinct experiments. *p < 0.05 as compared to mast cells preincubated in the absence of chemotactic stimuli