Fig. 2

Effect of r-Nef on chemotaxis of human basophils and mast cells. a Basophils were allowed to migrate toward r-Nef protein (3-300 ng/ml) for 1 h at 37 °C in the humidified incubator with 5% CO₂. Values are the mean ± SEM obtained from six independent experiments with different human basophil preparations. *p < 0.05 as compared to control. b Basophils were allowed to migrate toward the indicated concentrations of CXCL12/SDF-1α (white histogram), r-Nef (black histogram), and fMLF (grey histogram) for 1 h at 37 °C in a humidified incubator with 5% CO₂. Values are the mean ± SEM obtained from four experiments. *p < 0.05 as compared to control. c HLMC cells were allowed to migrate toward r-Nef protein (3-300 ng/ml) for 3 h at 37 °C in the humidified incubator with 5% CO₂. Values are the mean ± SEM obtained from six different experiments. *p < 0.05 as compared to control