Fig. 1From: HIV-1 Nef promotes migration and chemokine synthesis of human basophils and mast cells through the interaction with CXCR4CXCR4 expression on human basophils and mast cells. a Cytofluorimetric analysis of CXCR4 expression by human basophils purified from normal donors, HIV-1 and HIV-2 seronegative. Basophils were incubated (25 °C, 45 min) with monoclonal anti-CXCR4 PerCP-labelled (5 µg/ml) and anti-IgE FITC-labelled (white histogram) or isotype-matched antibodies (grey histogram). b Cytofluorimetric analysis of CXCR4 expression by HLMC cells purified from normal donors, HIV-1 and HIV-2 seronegative. Mast cells were incubated (25 °C, 45 min) with monoclonal anti-CXCR4 PerCP-labelled (5 µg/ml) and anti-IgE FITC-labelled (white histogram) or isotype-matched antibodies (grey histogram). c Mean fluorescence intensity of CXCR4 expression in basophils (grey column) and HLMC cells (black column) over basalBack to article page