Fig. 5

Soluble HLA-G5 mediates the development of Th17/Th2 cells by increasing IL-4 and IL-17A production by the CD4+ T helper cells. a IL-4, IL-17A, IL-17F and IFN-γ (mean ± SEM) were measured by bead-based assays in the supernatants of SK-specific T cell lines derived in the presence and in the absence of HLA-G5. b mRNA levels (mean ± SEM) of IL-4, IL-17A, IL-17F, IL-23R and IFN-γ were measured by RT-PCR in SK-specific T cell lines derived in the presence and in the absence of HLA-G5. c CD4+ T cell clones were derived from the 4 SK-specific T cell lines derived in the presence of HLA-G5 (N = 87) and from the 4 SK-specific T cell lines derived in the absence of HLA-G5 (N = 68) of 4 donors and the levels of IFN-γ, IL-4, IL-22, IL-17A and IL-17F produced by the T cell clones were measured. The percentages of Th17/Th1 and Th17/Th2 and Th17/Th0 and pure Th17 CD4+ T cell clones derived from the SK-TCL modulated in presence or absence of HLA-G5 were analysed. Data are represented as mean ± SEM. d The levels of IL-4, IL-17A, IL-17F and IL-22 produced by the Th17/Th2 T cell clones in the SK-specific T cell lines derived in the absence and in the presence of HLA-G5 were measured by bead based assays. Data are represented as mean ± SEM. For a, b and d the statistical analysis was performed with Wilcoxon test. For c the statistical analysis was performed with Chi-square test