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Table 1 Subjects’ selection criteria

From: Skin microbiota of first cousins affected by psoriasis and atopic dermatitis

Subjects’ characteristics
Sex Male
Age 50–53 years old
Subjects relationship All individuals were first cousins
Diet A Mediterranean diet was followed for 1 month before the day sampling. Pollen- and allergen- associated food, such as apple, hazelnut, celery, strawberries, shellfish and read meat were excluded from diet. Furthermore, milk intake was not recommended. Foods and beverages allowed were: bread, potatoes, vegetables, fresh fruit, meat and meat products, fish and fish products, eggs, edible fat, coffee, tea and soft drinks
Lifestyle All individuals lived in the same neighborhood. No sport or daily exercises were practiced during the study period. No travel or excursion were carried out for at least 1 month before the day of sampling
Occupation All subjects had a sedentary office work
Sexual activity No sexual activity for 2 weeks before the day of sampling
Clothing All individuals used only cotton clothes for all the study period
Pharmacological therapy No antibiotic therapy was administered for at least 1 month before the day of sampling and they were not subjected to any kind of pharmacological therapy
Probiotic therapy No probiotic therapy was administered for at least 1 month before the day of sampling
Personal care All subjects used Cetaphil, a free-preservatives soap, once a day for 1 month before the day of sampling. Cetaphil is an oil-in-water petrolatum-based cream used to treat dry skin and often recommended for the management of AD. Moreover, no skin perfume or cream was used during the study
Others No allergy to food, dust, pollen, grasses and drugs was present
The subjects enrolled in the study shared no genetic diseases
All subjects had not pets and/or contacts with any kind of animals
All individuals were nonsmokers and not subjected to passive smoking
The day of sampling took place the same day for all subjects enrolled in the study. The study was conducted during the winter season, in order to avoid excessive sweating