Figure 3

CD4+ CD161+ T cells producing IL-17 accumulate in HS lesion and gut. (A) CXCR3 and CD161 expression in CD4+ T lymphocytes obtained from PB and HS lesion of the three patients with HS associated with CD. Symbols represents each single patient. *p < 0.05. (B) Cytokines production profile of CD4+ T lymphocytes obtained from PB and HS lesions of the three patients with HS associated with CD. Symbols represents each single patient. (C) Flow cytometric plots intracellular staining of IL-17 and IFN-γ cytokines of CD4+ T lymphocytes after in vitro polyclonal stimulation, obtained from indicated tissues. One donor is represented. (D) Three representative TCR-BV families (TCR-BV3; TCR-BV5.1; TCR-BV22) in HS lesion zones, blood and gut of one patient with HS associated to CD. Asterisks indicate the presence of clonotypes found expanded in the peaks of interest.