Figure 4

The mRNA expressions and protein secretion of IL-5 by ALBE in primary murine splenocytes. (A) The effects of ALBE on the mRNA expression of IL-5. ALBE (100 μg/ml) were treated to splenocytes with or without ConA (3 μg/ml) for 16 h. The mRNA expression of IL-5 was assessed by RT-PCR described in method. Each bar shows the means ± SEM of three independent experiments. (B) The effects of ALBE on the protein secretion of IL-5. ALBE (100 μg/ml) were treated to splenocytes with or without ConA (3 μg/ml) for 16 h. The protein secretion of IL-5 was assessed by ELISA described in methods. Each bar shows the means ± SEM of four independent experiments. ^##P < 0.01: significantly different from the untreated group. **P < 0.01: significantly different from the ConA alone group.