Allergen specific IgE and IgG4 from RIT patients evaluated over time. Serum from each of the RIT patients was collected at various timepoints, pre-RIT, at the 16 week (4 month) visit before RIT was administered and at the 52 week (12 month) visit before treatment. Allergen specific IgE levels (kU [A]/L) were assessed by the ImmunoCAP250 method. Allergen specific IgG4 levels (mg [A]/L) were assessed by the UniCap100 method. Aeroallergens included Bermuda grass (green circle), Ragweed (brown square), D. pteronyssinus (red up-triangle) and D. farinea (blue down-triangle).