Figure 4From: IgG transmitted from allergic mothers decreases allergic sensitization in breastfed offspringFcRn expression was required for offspring to obtain the protective factor(s) from breast milk. Pups are those identified in Table 1. Following weaning, allergic airway disease was elicited in 8 week old offspring by two immunizations with OVA-Al(OH)3 followed by challenge for 4 days with 1% aerosolized OVA (daily exposure time 60 min). Parameters of disease severity measured were (A) OVA-specific IgE titers determined by ELISA; and (B and C) distribution of leukocyte populations in the airways determined by fluorescence flow cytometry. (D) Representative lung sections after OVA-aerosol challenge. Results expressed as means ± SEM and represent 12-19 mice per group. * p ≤ 0.05, ** p ≤ 0.01, or *** p ≤ 0.001 when compared to FcRn+/-/B6naive offspring (positive controls for disease) similarly subjected to AAD. Similar differences in OVA-specific IgE titers and histopathology between groups were obtained in an independent experiment.Back to article page