Figure 3

Effect of cAMP on allergen gene expression. Relative expression levels on AMM + 1% glucose are shown; 1 mM of dbtcAMP was added to the growth medium and the expression profiles of 17 A. fumigatus allergen genes were assessed by qRT-PCR. The results were normalised against the β-tubulin gene and compared to the expression of the same allergen genes in the medium lacking dbtcAMP. Expression of 16/17 allergen genes (except Asp f 23) was repressed by the presence of dbtcAMP; +cAMP, expression level on AMM + 1% glucose + 1 mM dbtcAMP; T, expression of the β-tubulin comparator. Error bars represent standard error of mean of biological replicates calculated using GraphPad PRISM 4.0.