Wortmannin dose-response in anti-IgE activated basophils, by assaying CD63 MFI (A), CD63expr % (B) CD203c MFI (C) and CD123 MFI (D). Basophils were pre-treated 10 min at 37°C with increasing doses of wortmannin and subsequently stimulated for further 30 min at 37°C with 4 μg/ml anti-IgE. Values are mean ± S.E.M. of triplicate assays of 2 separate experiments performed. Negative and positive controls as in legend of Figure 4. One way global ANOVA for activated samples: A) p < 0.0001; B) p < 0.0001; C) p < 0.001; D) p = 0.76. Post-hoc (LSD) analysis of each dose as compared to positive control is indicated above the respective bar chart as: (*) p < 0.05; (***) p < 0.001.