Figure 2

Dose response of quercetin on membrane markers expression by calcium ionophore (A,B,C) and PMA (D,E,F) activated basophils. Cells were pre-treated for 10 min at 37°C with increasing doses of quercetin, then stimulated for further 30 min at 37°C with 0.5 μM calcium ionophore A23187 or 50 nM PMA, then evaluated as CD63 MFI (A, D), as the percentage of cells expressing CD63 marker (B, E) and as CD203c MFI (C, F). Values are mean ± S.E.M. of triplicate assays. Figure is representative of one triplicate experiment of 4 performed.