An extract of the medicinal mushroom Agaricus blazei Murill can protect against allergy

Table 1 Scheme for experimental design in murine allergy model

Exp #	# Mice, strain	Treatment before and/or after OVA immunization			Harvest
1	16 NIH/OlaHsd	AndoSan™ or PBS p.o.* (200 μl) ↓			serum, spleen ↑
2	16 NIH/OlaHsd			AndoSan™/PBS p.o. ↓	serum, spleen ↑
3	8 C57Bl/6	AndoSan™/PBS p.o. and/or ↓		AndoSan™/PBS p.o. ↓	serum, spleen ↑
4	8 Balb/c	AndoSan™/PBS s.c. in foot pad (20 μl)** ↓			serum, PLN ↑
Day	-1	0	19,	20	26
Immunization		↑ OVA (10 μg) + Al(OH)3 s.c. in tailbase or foot pad +		↑ OVA s.c	↑ Sacrifice

*200 μl of AndoSan™ or PBS was given p.o. via a gastric catheter. ** OVA and Al(OH)₃ (2 mg) was dissolved in AndoSan™ or PBS before s.c. injection in foot pad.

ISSN: 1476-7961