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Table 2 Comparison of patients' IgE binding to rCyn d 1 and deletion mutants.

From: Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP)

Sera rCyn d 1 D1 D2 D3
P1 100 47.7 59 29.5
P2 100 64.3 57 31.4
P3 100 59.1 68 30.3
P4 100 45.5 64.9 28.6
Pool n = 10 100 54.2 58.3 33.3
Mean ± SEM   54.2 ± 3.5 61.4 ± 2.1 30.6 ± 0.8
  1. The percentages of IgE binding of deletion mutants relative to whole rCyn d 1 are shown for individual serum (P1–P4) and pooled patient sera (n = 10) as determined by ELISA. The percentage binding was calculated using the OD readings obtained through direct ELISA. The OD reading with whole purified rCyn d 1 was expressed as 100% and the OD of the deletion mutants were expressed in percentage relative to that of whole rCyn d 1. The mean ± SEM is shown in the bottom line.