Sera | rCyn d 1 | D1 | D2 | D3 |
|---|
P1 | 100 | 47.7 | 59 | 29.5 |
P2 | 100 | 64.3 | 57 | 31.4 |
P3 | 100 | 59.1 | 68 | 30.3 |
P4 | 100 | 45.5 | 64.9 | 28.6 |
Pool n = 10 | 100 | 54.2 | 58.3 | 33.3 |
Mean ± SEM | | 54.2 ± 3.5 | 61.4 ± 2.1 | 30.6 ± 0.8 |
- The percentages of IgE binding of deletion mutants relative to whole rCyn d 1 are shown for individual serum (P1–P4) and pooled patient sera (n = 10) as determined by ELISA. The percentage binding was calculated using the OD readings obtained through direct ELISA. The OD reading with whole purified rCyn d 1 was expressed as 100% and the OD of the deletion mutants were expressed in percentage relative to that of whole rCyn d 1. The mean ± SEM is shown in the bottom line.
