Figure 4

Dot blot immunoscreening of the seven overlapping fragments (F1–F7), whole rCynd 1 and deletion mutants. Dot blot immunoscreening of the seven overlapping fragments (F1–F7), whole rCynd 1 (F8) and deletion mutants (D1, D2, D3) with pooled sera from 10 patients and four individual patients with BGP allergy. Purified recombinant fusion proteins were applied onto PVDF membrane at 3 different concentrations (300 nM, 400 nM and 500 nM) and PBS buffer as negative control (NC).