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Table 2 Protein identification after 2DE-separation of proteins in sugar beet pollen extract

From: The identification of allergen proteins in sugar beet (Beta vulgaris) pollen causing occupational allergy in greenhouses

Sample Rank Protein Name Acc Nr MW pI #
1 1 CALM1_PETHY Calmodulin-1 (CaM-1) – Petunia hybrida P62199 16 763 4.1 6
2 1 CALM1_PETHY Calmodulin-1 (CaM-1) – Petunia hybrida P62199 16 763 4.1 5
3 1 PROF_CUCME Profilin (Pollen allergen Cuc m 2) – Cucumis melo (Muskmelon) Q5FX67 14 029 4.6 2
4 1 PROF_CUCME Profilin (Pollen allergen Cuc m 2) – Cucumis melo (Muskmelon) Q5FX67 14 029 4.6 2
5 1 CHE1_CHEAL Pollen allergen Che a 1 – Chenopodium album (Lamb's-quarters) Q8LGR0 18 739 5.0 2
  2 SODC1_MESCR Superoxide dismutase – Mesembryanthemum crystallinum P93258 15 278 5.5 1
6 1 PMGI_MESCR 2,3-bisphosphoglycerate-independent phosphoglycerate mutase Q42908 61 316 5.4 1
  2 SODC1_MESCR Superoxide dismutase – Mesembryanthemum crystallinum P93258 15 278 5.5 1
  4 TRXH1_TOBAC Thioredoxin H-type 1 – Nicotiana tabacum P29449 14 118 5.6 1
  5 CHE1_CHEAL Pollen allergen Che a 1 – Chenopodium album (Lamb's-quarters) Q8LGR0 18 739 5.0 3
7 1 DYH1A_CHLRE Dynein-1-alpha heavy chain – Chlamydomonas reinhardtii Q9SMH3 52 5420 5.3 1
8 1 CALM1_PETHY Calmodulin-1 (CaM-1) – Petunia hybrida P62199 16 763 4.1 2
  1. Samples were excised from 2DE (see Fig. 4A) and analyzed by LC-MS/MS. Protein identification after LC-MS/MS was performed with the software GPS Explorer and an in-house Mascot search engine. Settings: Precursor Tol: 15 ppm, MS/MS Fragment Tol: 0.15 Da. For each protein identification, columns show: Accession number in the Swiss-Prot database (Acc Nr), Protein theoretical mass in Da (MW), Protein theoretical isoelectric point (pI), and the number of peptides used for protein identification (#). The Mascot Best ion score (i.e. the highest score of a single peptide), and the significance of the database search (C.I. = the confidence interval) calculated by the GPS Explorer software were > 45 with at a confidence interval (C.I.) > 99.0 for all peptides except for Che a 1 in sample 6 where Best ion score was 17 and C.I. = 90.1%. Best ion score was 56 and C.I. = 99.999% for the Che a 1-homologue in sample 5. Best ion core was 82 and 62 with C.I. = 100% for the Che a 2-homologue in sample 3 and 4.