Skip to main content
Figure 1 | Clinical and Molecular Allergy

Figure 1

From: The identification of allergen proteins in sugar beet (Beta vulgaris) pollen causing occupational allergy in greenhouses

Figure 1

Separation of proteins in sugar beet pollen extract. Sugar beet pollen extract loaded corresponding to a protein content of 4 μg (lane 1), 20 μg (lane 2) and 50 μg (lanes 3 and 8). A. SDS-PAGE with standard gel, 12% polyacrylamide. B. SDS-PAGE with high-resolution gel, 15% polyacrylamide, giving better resolution in the mass range < 20 kDa. For reference, the well-characterized allergen in apple (Mal d 1, 1.5 μg, lane 4), and the three recombinant Chenopodium allergens are indicated by arrows, Che a 1 (lane 5), Che a 2 (lane 6) and Che a 3 (lane 7, with carry-over of material from lane 8). Gels stained with CBB. The calculated molecular masses of the allergens are 17.5 kDa (Mal d 1), 18 kDa (Che a 1), 14 kDa (Che a 2), 10 kDa (Che a 3).

Back to article page

Clinical and Molecular Allergy

ISSN: 1476-7961

Contact us