Figure 1

Human basophils gating with the BD-FACScanto flow cytometer. Leukocytes are immunologically gated in the CD45^dim-lymphocyte area and electronically captured as cellular events in HLADR^{non expressing}/CD123^bright biparametric logarithmic plot to distinguish them from other events and identified as basophils from monocytes (Q1), plasmacytoid dendritic cells (Q2) and lymphocytes (Q3). (A) Resting cells, (B) activation with 1 μg/ml goat anti-human IgE.