Figure 1
From: Diversity of the gut microbiota and eczema in early life
Denaturating gradient gel electrophoresis (DGGE). Stool samples were processed to extract 16S rDNA, the V2–V3 region was amplified by PCR, and denaturating gradient gel electrophoresis (DGGE) was performed using a standard protocol. For each lane, representing a single sample, the number of bands was counted and the Shannon index of diversity H' was calculated.