Effects of BAI on IκBα proteins levels in cytoplasm of IL-1β- and TNF-α-activated HMC-1 cells. HMC-1 cells were cultured with IL-1β or TNF-α in the presence or absence of BAI (30 μM) for 24 hours. Cytoplasmic extracts were prepared from each sample, and levels of IκBα proteins were analyzed by Western blot. BAI co-cultured with IL-1β (panel A) and TNF-α (panel B) showed markedly increased intensities when compared with the IL-1β or TNF-α alone. Densitometric analysis of IκBα was expressed as integrated intensity and shown in the bar graph.