RT-PCR analysis of effects of BAI on the gene expression of IL-6, IL-8, and MCP-1 in IL-1β- and TNF-α-activated HMC-1 cells. HMC-1 cells were treated with: A. IL-1β (10 ng/ml) with and without BAI (30 μM), and B. TNF-α (100 U/ml) with and without BAI (30 μM) for 6 hours before harvested for RNA preparation. RNA was subjected to RT-PCR with specific primers for target genes. HPRT was used as a house keeping gene to ensure equal loading. There were mild decreased gene expressions in IL-6 and MCP-1 and mild increased in IL-8 by BAI co-cultured with IL-1β-activated HMC-1 (Panel A). However, markedly decreased gene expressions of IL-6, IL-8, and MCP-1 were showed by BAI co-cultured with TNF-α-activated HMC-1 (Panel B). By densitometric analysis, the ratio of the expression of cytokine to HPRT was calculated and assigned as the intensity index as shown in the bar graph.