Effects of Baicalein (BAI) on production of IL-6, IL-8, and MCP-1 from IL-1β-activated HMC-1 cells. To each well of a 6-well culture plate, two ml of HMC-1 (1 × 106 cells/ml) were cultured alone (Control), or in the presence of BAI (30 μM), IL-1β (10 ng/ml), and the combinations of IL-1β (10 ng/ml) with different concentrations of BAI (1.8 to 30 μM) for 24 hrs in triplicate. Supernatants were harvested for measuring IL-6, IL-8, and MCP-1 by ELISA. The IL-6 (Panel A), IL-8 (Panel B), and MCP-1 (Panel C) production was significantly decreased when BAI was added in IL-1β-activated HMC-1 cells. *, +, and # indicate p < 0.05, <0.0005, and <0.00005, respectively, when compared with the IL-1β-treated group.