Table 2 Chemokine receptor expression in memory T helper cells induced by 7 days of antigenic stimuli. Median chemokine receptor expression in memory Th cells in percentage and MFI after 7 days of stimulation with antigen (allergen (15 μg/ml) or TTx (10 μg/ml)) or no antigen as a control. n = 10 for the healthy controls, n = 10 for the asymptomatically sensitized individuals and n = 8 for the allergic individuals except for CCR8 where n = 6 for the asymptomatically sensitized and allergic individuals. 10.000 PBMCs were acquired for the analysis. Isotype control cut-off values were set to >98%. Samples were run in monocates. For experimental design and analysis see Methods.

CCR3

Allergic

10.6 (4.7–32)

17.3 (9.8–29.5)

15.6 (4.4–52.2)

21 (16.2–32.3)

19.4 (16.9–37.2)

26.1* (16.6–40.5)

AS

10.3 (3.3–19.7)

8.7 (2.8–23.2)

11.9 (5–28.8)

20.5 (14.7–27.2)

18.6 (16.4–32.3)

23.4 * (18.4–37.4)

Healthy

8.7 (1.5–45)

11.2 (3.2–36.8)

23.4 (4.5–44.3)

19.7 (12.6–45.4)

17.8 (12.5–32.2)

23.7 (12.5–38.4)

CCR5

Allergic

21.3 (7.8–31.5)

25 (4.1–53.9)

28.3 (12.3–61.1)

21.4 (16.8–27.9)

25.9 * (22.4–48.5)

26.1 (16.1–42.5)

AS

15.2 (2.6–36.6)

17 (3–33.5)

16.9 * (5.2–65.9)

18.3 (15.2–24.5)

17.5 (15.4–20.5)

22.5 * (18.2–58.2)

Healthy

11.7 (5.6–34.5)

13.5 * (6.7–29.7)

20.2 * (10.1–74.1)

16.7 (12.7–22)

17.3 (13.5–25.6)

25.1 * (13.4–72)

CCR8

Allergic

10.4 (1.6–20.4)

12.8 (3.3–20)

12.7 (2.2–27.4)

24.3 (16.8–37.6)

27.9 (18.8–40.3)

28.2 (17.8–82.6)

AS

3.7 (1.8–16.6)

3.2 (1.7–14.7)

6.3 (1.4–34.9)

21.9 (17–26.1)

22.8 (17.2–44.6)

24.9 (16.2–36.6)

Healthy

5.7 (0.7–16.4)

3.9 (1.5–14)

9.3 * (3.8–35.7)

19 (12.6–49.6)

19.2 (11.7–46.6)

23.9 (12.4–48.2)

CXCR3

Allergic

42.9 (22–47.5)

42.1 (19.2–67.4)

49.2 * (27–74.2)

57.4 (33.1–65.3)

54.4 (48.4–72.4)

60.6 (36.9–81.8)

AS

28.1 (18–56.6)

27.8 (21–56.8)

29 * (20.8–77.4)

39.7 (29.3–59.7)

42.3 (30.6–54.5)

46.5 (33.5–115)

Healthy

28.8 (19.8–46.5)

31.4 (17.3–46.9)

35.9 * (25.8–82.9)

37.8 (32–65.2)

39.3 (30.1–63.9)

57.6 * (32–162.4)