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Table 2 Chemokine receptor expression in memory T helper cells induced by 7 days of antigenic stimuli. Median chemokine receptor expression in memory Th cells in percentage and MFI after 7 days of stimulation with antigen (allergen (15 μg/ml) or TTx (10 μg/ml)) or no antigen as a control. n = 10 for the healthy controls, n = 10 for the asymptomatically sensitized individuals and n = 8 for the allergic individuals except for CCR8 where n = 6 for the asymptomatically sensitized and allergic individuals. 10.000 PBMCs were acquired for the analysis. Isotype control cut-off values were set to >98%. Samples were run in monocates. For experimental design and analysis see Methods.

From: CCR3, CCR5, CCR8 and CXCR3 expression in memory T helper cells from allergic rhinitis patients, asymptomatically sensitized and healthy individuals

   % median (range) MFI median (range)
   No Ag Allergen TTx No Ag Allergen TTx
CCR3 Allergic 10.6 (4.7–32) 17.3 (9.8–29.5) 15.6 (4.4–52.2) 21 (16.2–32.3) 19.4 (16.9–37.2) 26.1* (16.6–40.5)
  AS 10.3 (3.3–19.7) 8.7 (2.8–23.2) 11.9 (5–28.8) 20.5 (14.7–27.2) 18.6 (16.4–32.3) 23.4 * (18.4–37.4)
  Healthy 8.7 (1.5–45) 11.2 (3.2–36.8) 23.4 (4.5–44.3) 19.7 (12.6–45.4) 17.8 (12.5–32.2) 23.7 (12.5–38.4)
CCR5 Allergic 21.3 (7.8–31.5) 25 (4.1–53.9) 28.3 (12.3–61.1) 21.4 (16.8–27.9) 25.9 * (22.4–48.5) 26.1 (16.1–42.5)
  AS 15.2 (2.6–36.6) 17 (3–33.5) 16.9 * (5.2–65.9) 18.3 (15.2–24.5) 17.5 (15.4–20.5) 22.5 * (18.2–58.2)
  Healthy 11.7 (5.6–34.5) 13.5 * (6.7–29.7) 20.2 * (10.1–74.1) 16.7 (12.7–22) 17.3 (13.5–25.6) 25.1 * (13.4–72)
CCR8 Allergic 10.4 (1.6–20.4) 12.8 (3.3–20) 12.7 (2.2–27.4) 24.3 (16.8–37.6) 27.9 (18.8–40.3) 28.2 (17.8–82.6)
  AS 3.7 (1.8–16.6) 3.2 (1.7–14.7) 6.3 (1.4–34.9) 21.9 (17–26.1) 22.8 (17.2–44.6) 24.9 (16.2–36.6)
  Healthy 5.7 (0.7–16.4) 3.9 (1.5–14) 9.3 * (3.8–35.7) 19 (12.6–49.6) 19.2 (11.7–46.6) 23.9 (12.4–48.2)
CXCR3 Allergic 42.9 (22–47.5) 42.1 (19.2–67.4) 49.2 * (27–74.2) 57.4 (33.1–65.3) 54.4 (48.4–72.4) 60.6 (36.9–81.8)
  AS 28.1 (18–56.6) 27.8 (21–56.8) 29 * (20.8–77.4) 39.7 (29.3–59.7) 42.3 (30.6–54.5) 46.5 (33.5–115)
  Healthy 28.8 (19.8–46.5) 31.4 (17.3–46.9) 35.9 * (25.8–82.9) 37.8 (32–65.2) 39.3 (30.1–63.9) 57.6 * (32–162.4)
  1. Bold text and * indicates significant differences between the antigen (allergen or TTx) stimulated samples and control samples where no antigen was added.
  2. Ag: antigen AS: asymptomatically sensitized individuals.