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Figure 5 | Clinical and Molecular Allergy

Figure 5

From: House dust mite major allergens Der p 1 and Der p 5 activate human airway-derived epithelial cells by protease-dependent and protease-independent mechanisms

Figure 5

[Ca2+]i measurements in 106 A549 cells. 5 × 107 A549 cells were loaded with 2 μM indo-1/AM (see Materials and Methods), followed by washing and dual-wavelength measurement of fluorescence, using 106 cells/ml per measurement. [Ca2+]i was calculated as described. The three traces presented are representative for three independent experiments and show the effects of House dust mite extract (HDM), recombinant Der p 1 and recombinant Der p 5 on intracellular Ca2+ homeostasis. Arrows indicate when the compounds were added.

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