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Figure 3 | Clinical and Molecular Allergy

Figure 3

From: House dust mite major allergens Der p 1 and Der p 5 activate human airway-derived epithelial cells by protease-dependent and protease-independent mechanisms

Figure 3

Effects of the several protease inhibitors on IL-6 and IL-8 protein secretion by A549 cells. Cells were stimulated during 24 hours with an optimal concentration of recombinant (r)Der p 1 (20 μg/ml), in absence and presence of optimal inhibitory concentrations of chymostatin (50 μg/ml; serine-protease inhibitor), E64 (10 μM; cysteine-protease inhibitor), or PMSF (0.25 mM; serine-protease inhibitor), or a combination of E64 plus PMSF. IL-6 protein levels are expressed as pg/ml, whereas IL-8 protein levels are expressed as ng/ml. * p < 0.05, significantly enhanced expression compared to unstimulated cells (medium); # p < 0.05 significantly diminished expression compared to rDer p 1.

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