Ponceau S staining and immunoblot analysis of recombinant B. bassiana. Immunoblots were probed with individual sera, A) and B) as indicated on the panels using a 1:5 final concentration of sera. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, and 4) Bb-Hex. Panel 1) represents Ponceau S staining of the PVDF membrane after transfer.