Figure 4From: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassianaImmunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pooled from (10 each) patients displaying mold allergies as indicated on the panels (A-J, and K-T). The final concentration of individual sera in each pool was 1:35. An HRP conjugated goat anti-human IgE antibody was used as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, 4) Bb-Hex. Lane 5) 40 μg crude B. bassiana extract.Back to article page