Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pooled from (10 each) patients displaying mold allergies as indicated on the panels (A-J, and K-T). The final concentration of individual sera in each pool was 1:35. An HRP conjugated goat anti-human IgE antibody was used as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, 4) Bb-Hex. Lane 5) 40 μg crude B. bassiana extract.