Skip to main content
Figure 3 | Clinical and Molecular Allergy

Figure 3

From: The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives

Figure 3

Identification of CRTH2 expressing cells by flow cytometry. Left histogram : ungated leukocytes biparametric representation on the basis of side scatter characteristics (SSC, Y axis) and FITC-CRTH2 (X axis). Two CRTH2 expressing cell populations are easily distinguishable: the one with high light scatterings corresponds to the eosinophil population; the second one (gating region: A) comprises Th2 lymphocytes and basophils. Right histogram: cells from the gating region (A) expressed on the basis of PE-CD203c (X axis) and PC5-CD3 (Y axis) characteristics. Th2 lymphocytes were readily separated from basophils based on their positive CD3 expression while activated basophils express high levels of CD203c without expressing CD3.

Back to article page