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Table 2 IL-4/GM-CSF Combination Increases Protein Content in huASMC.

From: Research Upregulation of CD23 (FcεRII) Expression in Human Airway Smooth Muscle Cells (huASMC) in Response to IL-4, GM-CSF, and IL-4/GM-CSF

Cytokine (nM)

mg/106 cells (n = 3)

BSA (vehicle)

1.17 ± 0.08

IL-4 (0.5)

1.18 ± 0.01

GM-CSF

1.12 ± 0.03

IL-4 (0.5)/GM-CSF (0.4)

1.39 ± 0.02 *

  1. Alpha-smooth muscle isoactin positive huASMC (Clonetics) in T-75 flasks were starved for 24 h in 0.1% FBS containing medium M199. The cells were then stimulated with BSA (1 μg/ml)), IL-4 (0.5 nM), GM-CSF (0.4 nM), or IL-4/GM-CSF (0.4/0.5 nM) for 24 h. The cell lysates in RIPA buffer were analyzed for protein content using a commercially available BCA kit (Pierce). The optical density was read using a Bio-Kinetics EL-312 Microplate reader. Results are mean ± SEM (n = 3). *denotes value significantly different from the BSA vehicle treated control.
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Clinical and Molecular Allergy

ISSN: 1476-7961

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