Alpha-smooth muscle isoactin positive huASMC (Clonetics) in T-75 flasks were starved for 24 h in 0.1% FBS containing medium M199. The cells were then stimulated with BSA (1 μg/ml)), IL-4 (0.5 nM), GM-CSF (0.4 nM), or IL-4/GM-CSF (0.4/0.5 nM) for 24 h. The cell lysates in RIPA buffer were analyzed for protein content using a commercially available BCA kit (Pierce). The optical density was read using a Bio-Kinetics EL-312 Microplate reader. Results are mean ± SEM (n = 3). *denotes value significantly different from the BSA vehicle treated control.