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Table 1 Basophil cell yields. Average of numbers detected after lysis at 7 different concentrations of CRA1 from 250000 (normalized) events after lysis with either WBL or Immunoprep/Q-prep (average ± SD, tested with a paired t test). + = atopics, - = non atopics

From: Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI

Donor Allergy WBL ± SD Immunoprep ± SD p-value
1 - 1714 ± 314 1108 ± 449 <0,015
2 - 1979 ± 132 369 ± 274 <0,001
3 + 1638 ± 66 406 ± 262 <0,001
4 - 568 ± 84 182 ± 107 <0.001
5 - 1646 ± 282 371 ± 248 <0,001
6 + 483 ± 113 436 ± 138 0,494
7 - 1164 ± 70 213 ± 160 <0.001
8 - 1132 ± 372 582 ± 150 0,003
9 - 1397 ± 207 306 ± 133 <0,001
10 - 995 ± 481 577 ± 415 0,107
11 - 407 ± 128 397 ± 183 0,904
Median   1164 397