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Figure 4 | Clinical and Molecular Allergy

Figure 4

From: Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI

Figure 4

Comparison of response of CD63 and CD203c with different lysis methods. Degree of activation at varying concentrations of CRA1. The % positive cells at a given concentration are plotted against the amount of anti-FceRI antibody. The lowest data point is labelled 1e-8 for the purpose of representation on a log scale. (a) Immunoprep/Q-prep lysis, detection with CD63, (b) Immunoprep/Q-prep lysis, detection with CD203c, (c) WBL lysis, detection with CD63, (d) WBL lysis, detection with CD203c. In panel d, one responder achieved significantly higher activation ratios than 35.

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Clinical and Molecular Allergy

ISSN: 1476-7961

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