Figure 1From: Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRIAnalysis of Basophil activation. Analysis of basophils after lysis by Immunoprep/Q-prep (a–f) or WBL (g–l) of a representative donor. By dynamic backgating (illustrated in figure 2), the region in a forward scatter vs side scatter plot in which basophils are located was optimised (a & g). CD203c+ cells were identified in this gate (b & h), and the expression of CD203c and CD63 was evaluated (c & i). CD203c vs CD63 expression at differend concentrations of CRA1 is shown after both lysis conditions (1 ng/ul panels c, g, 0,001 ng/ul in panels f & l, 0, 0,0001 in panels e & k; pbs in panels d & j).Back to article page