Analysis of Basophil activation. Analysis of basophils after lysis by Immunoprep/Q-prep (a–f) or WBL (g–l) of a representative donor. By dynamic backgating (illustrated in figure 2), the region in a forward scatter vs side scatter plot in which basophils are located was optimised (a & g). CD203c+ cells were identified in this gate (b & h), and the expression of CD203c and CD63 was evaluated (c & i). CD203c vs CD63 expression at differend concentrations of CRA1 is shown after both lysis conditions (1 ng/ul panels c, g, 0,001 ng/ul in panels f & l, 0, 0,0001 in panels e & k; pbs in panels d & j).