RT-PCR analysis of effects of BAI on the gene expression of IL-6 and IL-8 in cigarette smoke extracts (CSE: Mainstream, Ms, and Sidestream, Ss)-treated and IL-1β- activated HMC-1 cells. HMC-1 were treated with IL-1β (10 μg/mL) and CSE in the presence or absence of BAI (30 μM) for 6 hours and harvested for transcriptional analysis via RT-PCR. The intensities of the cytokine and house keeping gene (HPRT) bands were measured by densitometry, and the ratio of the cytokine to the house keeping gene was calculated and assigned as the intensity index. IL-1β-treated HMC-1 increased IL-6 and IL-8 mRNA transcription. In the presence of Ms (0.125 mg/mL) or Ss (0.125 mg/mL) CSE, the expression of IL-6 and IL-8 was increased further. However, in the addition of BAI, the expression of IL-6 and IL-8 was remarkably decreased.