Defining the molecular role of gp91phox in the immune manifestation of acute allergic asthma using a preclinical murine model

Banerjee, Ena Ray; Henderson, William R

doi:10.1186/1476-7961-10-2

Table 1 Number of cells (× 10 ⁶ ) of leucocyte subsets in peripheral blood, lung parenchyma and bronchoalveolar lavage fluid (BALf).

From: Defining the molecular role of gp91phox in the immune manifestation of acute allergic asthma using a preclinical murine model

BLOOD	Leukocyte subsets (× 10⁶) in blood, lungs and BAL fluid post OVA treatment.
	Total WBCs	Lymphocytes		Monocytes	Neutrophils	Eosinophils	Basophils
		CD3+	B220+
WA	11.9 ± 2.4	5.54 ± 1.17	3.08 ± 1.05	0.38 ± 0.185	3.648 ± 0.184	0.223 ± 0.014	0.066 ± 0.001
WO	24.72 ± 6.41	7.31 ± 1.31	5.93 ± 1.17	1.62 ± 0.35	7.97 ± 1.14	0.74 ± 0.36	1.05 ± 0.02
NOXA	14.2 ± 3.012	5.7 ± 1.865	2.99 ± 1.033	0.77 ± 0.053	4.56 ± 1.074	0.133 ± 0.043	0.028 ± 0.002
NOXO	25.625 ± 4.063	6.186 ± 1.076	6.742 ± 0.964	2.424 ± 1.076	8.741 ± 0.064	1.061 * ± 0.004	0.556 ± 0.003
DKOA	11.7 ± 2.76	4.69 ± 1.06	2.467 ± 0.96	0.63 ± 0.12	3.74 ± 1.27	0.106 ± 0.07	0.02 ± 0.001
DKOO	27.56 ± 2.79	6.61 ± 1.49	7.27 ± 2.01	2.6 ± 0.47	9.39 ± 2.44	1.14 * ± 0.46	0.59 ± 0.14
Lungs
	Total	Lymphocytes		Monocytes	Neutrophils	Eosinophils	Basophils/	M ϕ
	WBCs	CD3+	B220+				Mast cells
WA	1.86 ± 0.543	0.179 ± 0.021	0.035 ± 0.002	0.826 ± 0.054	0.319 ± 0.054	0	0	0.497 ± 0.015
WO	12.35 ± 3.96	4.87 ± 1.05	0.09 ± 0.01	0.37 ± 0.09	1.47 ± 0.43	2.22 ± 0.76	0.046 ± 0.012	3.09 ± 1.08
NOXA	5.49 # ± 1.064	0.446 # ± 0.002	0.113 # ± 0.001	2.593 # ± 0.76	0.892 # ± 0.011	0	0	1.489 # ± 0.016
NOXO	22.6 * ± 7.544	4.084 ± 1.075	1.29 * ± 0.643	3.171 * ± 1.075	4.337 * ± 1.074	4.102 * ± 1.066	0.089 * ± 0.002	5.682 ± 0.064
DKOA	3.78 # ± 1.07	0.3 ± 0.14	0.07 ± 0.01	1.78 # ± 0.54	0.61 ± 0.02	0.001 ± 0.0001	0	1.02 # ± 0.29
DKOO	21.66 * ± 408	3.89 ± 1.06	0.43 * ± 0.16	3.03 * ± 0.89	4.16 * ± 1.33	3.93 * ± 1.13	0.08 * ± 0.002	5.44 * ± 1.26
BAL fluid (2 lungs)
WA	0.8 ± 0.013	0	0	0.013 ± 0.004	0	0	0	0.787 ± 0.011
WO	9.46 ± 0.89	1.64 ± 0.21	1.54 ± 0.021	1.26 ± 0.03	0.86 ± 0.01	4.26 ± 0.07	0.04 ± 0.002	0.56 ± 0.004
NOXA	0.63 ± 0.018	0	0	0.014 ± 0.002	0	0	0	0.616 ± 0.003
NOXO	16 * ± 3.159	2.04 ± 0.754	1.213 ± 0.003	2.08 ± 0.074	2.427 * ± 1.066	6.741 * ± 2.076	0.051 ± 0.001	1.568 * ± 0.72
DKOA	0.865 ± 0.01	0	0	0.025 ± 0.002	0	0	0	0.84 ± 0.18
DKOO	15.89 * ± 4.03	2 ± 0.86	1.22 ± 0.66	2.05 ± 0.75	2.415 *± 1.01	6.67 * ± 1.14	0.05 ± 0.003	1.55 * ± 0.63
Number of cells (× 10⁶) of leucocyte subsets in peripheral blood, lung parenchyma and bronchoalveolar lavage fluid (BALf).
		T cells	B cells	Monocytes	Neutrophils	Eosinophils	Basophils
Lungs	WO	0.6	0.015	0.22	0.18	3	0.04
	NOXO	0.6	0.19*	1.3	0.49*	3.8	0.16*
	DKOO	0.58	0.05	1.1	0.44*	3.4	0.13*
BALf	WO	0.22	0.25	0.77	0.107	5.75	0.03
	NOXO	0.32	0.17	0.85	0.27*	6.3	0.09
	DKOO	0.51	0.16	0.78	0.26*	5.8	0.08

Total white blood cells were counted by a Beckman Coulter particle counter and subsets quantified using specific antibody staining in FACS and corroborated by morphological assessment by light microscopy and also by hemavet using the specifics of mouse. The table shows data averaged from three independent experiments ± SEM. * denotes p value < 0.05 compared to values from post-OVA wildtype mice and # denotes p value < 0.05 compared to alum treated wildtype. Abbreviations used are: WA = wildtype +alum, WO = wildtype + OVA, NOXA = gp91phox-/- + alum, NOXO = gp91phox-/- + OVA, DKOA = MMP12-gp91phox double knockout + alum, DKOO = MMP12-gp91phox double knockout + OVA. (n = 5/group). Recruitment index: Inflammatory hematopoietic cells found in lungs and BALf expressed as a fraction of corresponding cells in circulation. Number of cells (× 10⁶) in lungs and BALf were the numerator and the number of cells (× 10⁶) in circulating blood was the denominator in the fraction calculated to show recruitment index or inflammatory cells recruited from circulation to lung parenchyma and airways in response to the OVA allergic challenge. * denotes statistically significant change (p value < 0.01)

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ISSN: 1476-7961

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