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Table 1 Number of cells (× 10 6 ) of leucocyte subsets in peripheral blood, lung parenchyma and bronchoalveolar lavage fluid (BALf).

From: Defining the molecular role of gp91phox in the immune manifestation of acute allergic asthma using a preclinical murine model

BLOOD Leukocyte subsets (× 106) in blood, lungs and BAL fluid post OVA treatment.
  Total
WBCs
Lymphocytes Monocytes Neutrophils Eosinophils Basophils   
   CD3+ B220+       
WA 11.9
± 2.4
5.54
± 1.17
3.08
± 1.05
0.38
± 0.185
3.648
± 0.184
0.223
± 0.014
0.066
± 0.001
  
WO 24.72
± 6.41
7.31
± 1.31
5.93
± 1.17
1.62
± 0.35
7.97
± 1.14
0.74
± 0.36
1.05
± 0.02
  
NOXA 14.2
± 3.012
5.7
± 1.865
2.99
± 1.033
0.77
± 0.053
4.56
± 1.074
0.133
± 0.043
0.028
± 0.002
  
NOXO 25.625
± 4.063
6.186
± 1.076
6.742
± 0.964
2.424
± 1.076
8.741
± 0.064
1.061 *
± 0.004
0.556
± 0.003
  
DKOA 11.7
± 2.76
4.69
± 1.06
2.467
± 0.96
0.63
± 0.12
3.74
± 1.27
0.106
± 0.07
0.02
± 0.001
  
DKOO 27.56
± 2.79
6.61
± 1.49
7.27
± 2.01
2.6
± 0.47
9.39
± 2.44
1.14 *
± 0.46
0.59
± 0.14
  
Lungs
  Total Lymphocytes Monocytes Neutrophils Eosinophils Basophils/ M ϕ  
  WBCs CD3+ B220+     Mast cells   
WA 1.86
± 0.543
0.179
± 0.021
0.035
± 0.002
0.826
± 0.054
0.319
± 0.054
0 0 0.497
± 0.015
 
WO 12.35
± 3.96
4.87
± 1.05
0.09
± 0.01
0.37
± 0.09
1.47
± 0.43
2.22
± 0.76
0.046
± 0.012
3.09
± 1.08
 
NOXA 5.49 #
± 1.064
0.446 #
± 0.002
0.113 #
± 0.001
2.593 #
± 0.76
0.892 #
± 0.011
0 0 1.489 #
± 0.016
 
NOXO 22.6 *
± 7.544
4.084
± 1.075
1.29 *
± 0.643
3.171 *
± 1.075
4.337 *
± 1.074
4.102 *
± 1.066
0.089 *
± 0.002
5.682
± 0.064
 
DKOA 3.78 #
± 1.07
0.3
± 0.14
0.07
± 0.01
1.78 #
± 0.54
0.61
± 0.02
0.001
± 0.0001
0 1.02 #
± 0.29
 
DKOO 21.66 *
± 408
3.89
± 1.06
0.43 *
± 0.16
3.03 *
± 0.89
4.16 *
± 1.33
3.93 *
± 1.13
0.08 *
± 0.002
5.44 *
± 1.26
 
BAL fluid (2 lungs)
WA 0.8
± 0.013
0 0 0.013
± 0.004
0 0 0 0.787
± 0.011
 
WO 9.46
± 0.89
1.64
± 0.21
1.54
± 0.021
1.26
± 0.03
0.86
± 0.01
4.26
± 0.07
0.04
± 0.002
0.56
± 0.004
 
NOXA 0.63
± 0.018
0 0 0.014
± 0.002
0 0 0 0.616
± 0.003
 
NOXO 16 *
± 3.159
2.04
± 0.754
1.213
± 0.003
2.08
± 0.074
2.427 *
± 1.066
6.741 *
± 2.076
0.051
± 0.001
1.568 *
± 0.72
 
DKOA 0.865 ± 0.01 0 0 0.025 ± 0.002 0 0 0 0.84 ± 0.18  
DKOO 15.89 *
± 4.03
2 ± 0.86 1.22 ± 0.66 2.05 ± 0.75 2.415 *± 1.01 6.67 *
± 1.14
0.05
± 0.003
1.55 *
± 0.63
 
Number of cells (× 106) of leucocyte subsets in peripheral blood, lung parenchyma and bronchoalveolar lavage fluid (BALf).
   T cells B cells Monocytes Neutrophils Eosinophils Basophils   
Lungs WO 0.6 0.015 0.22 0.18 3 0.04   
  NOXO 0.6 0.19* 1.3 0.49* 3.8 0.16*   
  DKOO 0.58 0.05 1.1 0.44* 3.4 0.13*   
BALf WO 0.22 0.25 0.77 0.107 5.75 0.03   
  NOXO 0.32 0.17 0.85 0.27* 6.3 0.09   
  DKOO 0.51 0.16 0.78 0.26* 5.8 0.08   
  1. Total white blood cells were counted by a Beckman Coulter particle counter and subsets quantified using specific antibody staining in FACS and corroborated by morphological assessment by light microscopy and also by hemavet using the specifics of mouse. The table shows data averaged from three independent experiments ± SEM. * denotes p value < 0.05 compared to values from post-OVA wildtype mice and # denotes p value < 0.05 compared to alum treated wildtype. Abbreviations used are: WA = wildtype +alum, WO = wildtype + OVA, NOXA = gp91phox-/- + alum, NOXO = gp91phox-/- + OVA, DKOA = MMP12-gp91phox double knockout + alum, DKOO = MMP12-gp91phox double knockout + OVA. (n = 5/group). Recruitment index: Inflammatory hematopoietic cells found in lungs and BALf expressed as a fraction of corresponding cells in circulation. Number of cells (× 106) in lungs and BALf were the numerator and the number of cells (× 106) in circulating blood was the denominator in the fraction calculated to show recruitment index or inflammatory cells recruited from circulation to lung parenchyma and airways in response to the OVA allergic challenge. * denotes statistically significant change (p value < 0.01)