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Figure 1 | Clinical and Molecular Allergy

Figure 1

From: Defining the molecular role of gp91phox in the immune manifestation of acute allergic asthma using a preclinical murine model

Figure 1

Study design to generate acute allergic asthma phenotype in mice: Mice were sensitized with OVA (100 μg) complexed with aluminium sulfate in a 0.2-ml volume, administered by i.p. injection on day 0 and later challenged with OVA On days 8 (250 μg of OVA) and on days 15, 18, and 21 (125 μg of OVA), (Pierce, Rockford, IL) by intra-tracheal instillation. Mice were immunized with OVA (100 μg) complexed with aluminium sulfate in a 0.2-ml volume, administered by i.p. injection on day 0. The control group received normal saline with aluminium sulfate by i.p. route on day 0 and 0.05 ml of 0.9% saline by i.t. route on days 8, 15, 18, and 21. They were sacrificed on d22. The abbreviations used are: i.p. intra-peritoneal; i.t. intra-tracheal; BAL, bronchoalveolar lavage; PB, peripheral blood.

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